Rabbit Recombinant Monoclonal NFE2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Western blot - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--western-blot-img146043.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105342.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95741.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577)")
![Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--flow-cytometry-intracellular-img26234.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (AB251577)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Alternative names
Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, p45 NF-E2, NFE2
Recommended products
Rabbit Recombinant Monoclonal NFE2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20463
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251577 is the carrier-free version of Anti-NFE2 antibody [EPR20463] ab223845.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--western-blot-img146043.jpg "Western blot - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)")
Western blot - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
This data was developed using Anti-NFE2 antibody [EPR20463] ab223845, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NFE2 antibody [EPR20463] (Anti-NFE2 antibody [EPR20463] ab223845) at 1/1000 dilution
Lane 1: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg
Lane 2: HEL (human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg
Lane 3: TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105342.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
This data was developed using Anti-NFE2 antibody [EPR20463] ab223845, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling NFE2 with Anti-NFE2 antibody [EPR20463] ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neutrophils (arrow) in human placenta (PMID: 8645254) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95741.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
This data was developed using Anti-NFE2 antibody [EPR20463] ab223845, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded malignant lymphoma of bone marrow tissue labeling NFE2 with Anti-NFE2 antibody [EPR20463] ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on a subset of cells of malignant lymphoma of bone marrow is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr20463-bsa-and-azide-free-ab251577--flow-cytometry-intracellular-img26234.jpg "Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)")
Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] - BSA and Azide free (ab251577)
This data was developed using Anti-NFE2 antibody [EPR20463] ab223845, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEL (human bone marrow erythroleukemia) cell line labeling NFE2 with Anti-NFE2 antibody [EPR20463] ab223845 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
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