Rabbit Recombinant Monoclonal NFE2 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-NFE2 antibody [EPR2824(2)] (AB140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--western-blot-img104450.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFE2 antibody [EPR2824(2)] (AB140598)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (AB140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122857.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (AB140598)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (AB140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81820.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (AB140598)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (AB140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54122.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (AB140598)")
Publications
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- Oncotarget 8:30138-301502017A novel role of CKIP-1 in promoting megakaryocytic differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiao Fan,Yiwu Wang,Yuan Shen,Qingyan Liu,Rong Gao,Ya Qiu,Chanjuan Wang,Lingqiang ZhangPubMed 28404913
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Tested | Expected |
| Mouse | Predicted | Expected | Not recommended | Predicted | Expected |
| Rat | Predicted | Expected | Not recommended | Predicted | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
Alternative names
Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, p45 NF-E2, NFE2
Recommended products
Rabbit Recombinant Monoclonal NFE2 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2824(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-NFE2 antibody [EPR2824(2)] (ab140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--western-blot-img104450.jpg "Western blot - Anti-NFE2 antibody [EPR2824(2)] (ab140598)")
Western blot - Anti-NFE2 antibody [EPR2824(2)] (ab140598)
All lanes: Western blot - Anti-NFE2 antibody [EPR2824(2)] (ab140598) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
Lane 4: U937 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122857.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598)
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling NFE2 with ab140598 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81820.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598)
Immunohistochemical analysis of paraffin embedded Human testis tissue labelling NFE2 with ab140598 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598), expandable thumbnail](https://content.abcam.com/products/images/nfe2-antibody-epr28242-ab140598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54122.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR2824(2)] (ab140598)
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling NFE2 with ab140598 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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