Anti-NFIB Antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFIB Antibody (AB327182)

Immunofluorescent staining of human cell line U-251 MG using ab327182 at 0.25–2 µg/ml, showing localization to nucleoplasm & nucleoli fibrillar center.

• ChIP

Supplier Data

ChIP - Anti-NFIB Antibody (AB327182)

ChIP-Exo-Seq composite graph for ab327182 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-NFIB Antibody (AB327182)

Western blot analysis in human cell line SH-SY5Y using ab327182 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-NFIB Antibody (ab327182)

All lanes:

Human cell line SH-SY5Y

Predicted band size: 47.44 kDa

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