Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) is a rabbit monoclonal antibody detecting NFIB / NF1B2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Images
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--western-blot-img95546.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738)")
![Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--flow-cytometry-intracellular-img26478.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738)")
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--western-blot-img81009.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738)")
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--western-blot-img106701.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17613.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (AB186738)")
Publications
Filter by
- Nature communications 14:50762023NFIB facilitates replication licensing by acting as a genome organizer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenting Zhang,Yue Wang,Yongjie Liu,Cuifang Liu,Yizhou Wang,Lin He,Xiao Cheng,Yani Peng,Lu Xia,Xiaodi Wu,Jiajing Wu,Yu Zhang,Luyang Sun,Ping Chen,Guohong Li,Qiang Tu,Jing Liang,Yongfeng ShangPubMed 37604829
- Stem cells international 2023:28268152023A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanghao Zhang,Xiaolong Xu,Luojiang Zhu,Sisi Li,Rundong Chen,Nan Lv,Zifu Li,Jing Wang,Qiang Li,Wang Zhou,Pengfei Yang,Jianmin LiuPubMed 37964983
- Development (Cambridge, England) 149:2022Dentate gyrus morphogenesis is regulated by β-catenin function in hem-derived fimbrial glia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpan Parichha,Debarpita Datta,Varun Suresh,Mallika Chatterjee,Michael J Holtzman,Shubha TolePubMed 36196585
- Signal transduction and targeted therapy 7:1762022Chimeric cerebral organoids reveal the essentials of neuronal and astrocytic APOE4 for Alzheimer's tau pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShichao Huang,Zhen Zhang,Junwei Cao,Yongchun Yu,Gang PeiPubMed 35691989
- Frontiers in molecular biosciences 9:8753242022Hepatocyte-Specific Knock-Out of Nfib Aggravates Hepatocellular Tumorigenesis via Enhancing Urea Cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Zhou,Qing-Liang Wang,Lin-Hong Mao,Si-Yuan Chen,Zi-Han Yang,Xue Liu,Yu-Hua Gao,Xiao-Qin Li,Zhi-Hang Zhou,Song HePubMed 35655758
- Journal of immunology research 2021:86751232021NFIB-Mediated lncRNA PVT1 Aggravates Laryngeal Squamous Cell Carcinoma Progression via the miR-1301-3p/MBNL1 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Tang,Feng ZengPubMed 34805417
- Cancer cell 39:1479-1496.e182021Signatures of plasticity, metastasis, and immunosuppression in an atlas of human small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph M Chan,Álvaro Quintanal-Villalonga,Vianne Ran Gao,Yubin Xie,Viola Allaj,Ojasvi Chaudhary,Ignas Masilionis,Jacklynn Egger,Andrew Chow,Thomas Walle,Marissa Mattar,Dig V K Yarlagadda,James L Wang,Fathema Uddin,Michael Offin,Metamia Ciampricotti,Besnik Qeriqi,Amber Bahr,Elisa de Stanchina,Umesh K Bhanot,W Victoria Lai,Matthew J Bott,David R Jones,Arvin Ruiz,Marina K Baine,Yanyun Li,Natasha Rekhtman,John T Poirier,Tal Nawy,Triparna Sen,Linas Mazutis,Travis J Hollmann,Dana Pe'er,Charles M RudinPubMed 34653364
- iScience 24:1029532021ASCL1, NKX2-1, and PROX1 co-regulate subtype-specific genes in small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarine Pozo,Rahul K Kollipara,Demetra P Kelenis,Kathia E Rodarte,Morgan S Ullrich,Xiaoyang Zhang,John D Minna,Jane E JohnsonPubMed 34466783
- PeerJ 9:e108482021NFIB promotes the migration and progression of kidney renal clear cell carcinoma by regulating PINK1 transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNinghua Wang,Jing Yuan,Fei Liu,Jun Wei,Yu Liu,Mei Xue,Rui DongPubMed 33981484
- Molecular medicine reports 23:2021NFIB promotes the progression of gastric cancer by upregulating circMAP7D1 to stabilize HER2 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuimin Yang,Zhengzhen Wu,Xin Liu,Min Chen,Xin Zhang,Yong JiangPubMed 33576439
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 - 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcriptional activator of GFAP, essential for proper brain development (PubMed:30388402). Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Alternative names
Nuclear factor 1 B-type, NF1-B, Nuclear factor 1/B, CCAAT-box-binding transcription factor, Nuclear factor I/B, TGGCA-binding protein, CTF, NF-I/B, NFI-B, NFIB
Recommended products
Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) is a rabbit monoclonal antibody detecting NFIB / NF1B2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14122
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of NFIB / NF1B2?
Anti-NFIB / NF1B2 [EPR14122] (ab186738) specifically detects a band for NFIB / NF1B2 (UniProt: O00712) at a molecular weight of 47kDa.
Trusted by the scientific community
Anti-NFIB / NF1B2 [EPR14122] (ab186738) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR14122] also available for your convenience:
ab186738, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-NFIB / NF1B2 antibody [EPR14122] ab216931, Carrier free - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free ab250892, PE - PE Anti-NFIB / NF1B2 antibody [EPR14122] ab305979, APC - APC Anti-NFIB / NF1B2 antibody [EPR14122] ab305980, HRP - HRP Anti-NFIB / NF1B2 antibody [EPR14122] ab305981, Alkaline Phosphatase - Alkaline Phosphatase Anti-NFIB / NF1B2 antibody [EPR14122] ab308716, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-NFIB / NF1B2 antibody [EPR14122] ab310047, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-NFIB / NF1B2 antibody [EPR14122] ab310434, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-NFIB / NF1B2 antibody [EPR14122] ab311961, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-NFIB / NF1B2 antibody [EPR14122] ab312433, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-NFIB / NF1B2 antibody [EPR14122] ab321300
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--western-blot-img95546.jpg "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
All lanes: Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution
All lanes: Human fetal brain at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--flow-cytometry-intracellular-img26478.jpg "Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--western-blot-img81009.jpg "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
All lanes: Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution
All lanes: HeLa cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa, 53 kDa, 55 kDa
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--western-blot-img106701.jpg "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
All lanes: Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/1000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: PC-12 cell lysate at 10 µg
Lane 3: NIH/3T3 cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17613.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunoprecipitation-img88771.jpg "Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
All lanes: Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57625.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunocytochemistry-immunofluorescence-img98339.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)
Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.
![Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunoprecipitation-img88771.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57625.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
![Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-ab186738--immunocytochemistry-immunofluorescence-img98339.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com