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AB250892

Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NFIB / NF1B2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

Nuclear factor 1 B-type, NF1-B, Nuclear factor 1/B, CCAAT-box-binding transcription factor, Nuclear factor I/B, TGGCA-binding protein, CTF, NF-I/B, NFI-B, NFIB

8 Images
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • WB

Supplier Data

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (<a href='/en-us/products/primary-antibodies/nfib-nf1b2-antibody-epr14122-ab186738'>ab186738</a>) at 1/5000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa,53 kDa,55 kDa

true

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • WB

Supplier Data

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (<a href='/en-us/products/primary-antibodies/nfib-nf1b2-antibody-epr14122-ab186738'>ab186738</a>) at 1/5000 dilution

All lanes:

Human fetal brain at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • IP

Unknown

Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (<a href='/en-us/products/primary-antibodies/nfib-nf1b2-antibody-epr14122-ab186738'>ab186738</a>)

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)
  • WB

Supplier Data

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)

This data was developed using ab186738, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (<a href='/en-us/products/primary-antibodies/nfib-nf1b2-antibody-epr14122-ab186738'>ab186738</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

PC-12 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14122

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250892 is the carrier-free version of ab186738.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator of GFAP, essential for proper brain development (PubMed : 30388402). Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
See full target information NFIB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:19689 PubMed31873158

2019

Gender-specific effects of transthyretin on neural stem cell fate in the subventricular zone of the adult mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Pieter Vancamp,Jean-David Gothié,Cristina Luongo,Anthony Sébillot,Karine Le Blay,Lucile Butruille,Maurice Pagnin,Samantha J Richardson,Barbara A Demeneix,Sylvie Remaud
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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