Rabbit Recombinant Monoclonal NFIB / NF1B2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--western-blot-img95546.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)")
![Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--flow-cytometry-intracellular-img26478.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)")
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--western-blot-img81009.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)")
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--western-blot-img106701.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17613.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (AB250892)")
Publications
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- Scientific reports 9:196892019Gender-specific effects of transthyretin on neural stem cell fate in the subventricular zone of the adult mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPieter Vancamp et. al.PubMed 31873158
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
8 products for Alternative Version
Target data
Function
Transcriptional activator of GFAP, essential for proper brain development (PubMed:30388402). Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Alternative names
Nuclear factor 1 B-type, NF1-B, Nuclear factor 1/B, CCAAT-box-binding transcription factor, Nuclear factor I/B, TGGCA-binding protein, CTF, NF-I/B, NFI-B, NFIB
Recommended products
Rabbit Recombinant Monoclonal NFIB / NF1B2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14122
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250892 is the carrier-free version of Anti-NFIB / NF1B2 antibody [EPR14122] ab186738.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--western-blot-img95546.jpg "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (Anti-NFIB / NF1B2 antibody [EPR14122] ab186738) at 1/5000 dilution
All lanes: Human fetal brain at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--flow-cytometry-intracellular-img26478.jpg "Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Flow Cytometry (Intracellular) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.
Anti-NFIB / NF1B2 antibody [EPR14122] ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--western-blot-img81009.jpg "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (Anti-NFIB / NF1B2 antibody [EPR14122] ab186738) at 1/5000 dilution
All lanes: HeLa cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa, 53 kDa, 55 kDa
![Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--western-blot-img106701.jpg "Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NFIB / NF1B2 antibody [EPR14122] (Anti-NFIB / NF1B2 antibody [EPR14122] ab186738) at 1/1000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: PC-12 cell lysate at 10 µg
Lane 3: NIH/3T3 cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17613.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with Anti-NFIB / NF1B2 antibody [EPR14122] ab186738 at a 1/500 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunoprecipitation-img88771.jpg "Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
All lanes: Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] (Anti-NFIB / NF1B2 antibody [EPR14122] ab186738)
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57625.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with Anti-NFIB / NF1B2 antibody [EPR14122] ab186738 at a 1/500 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunocytochemistry-immunofluorescence-img98339.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
This data was developed using Anti-NFIB / NF1B2 antibody [EPR14122] ab186738, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with Anti-NFIB / NF1B2 antibody [EPR14122] ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.
![Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunoprecipitation-img88771.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57625.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
![Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892), expandable thumbnail](https://content.abcam.com/products/images/nfib-nf1b2-antibody-epr14122-bsa-and-azide-free-ab250892--immunocytochemistry-immunofluorescence-img98339.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)")
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