AB245597

Anti-NFIC/CTF antibody

Name: Anti-NFIC/CTF antibody (ab245597) | Abcam
Brand: Abcam Limited
SKU: AB245597
Price: 530 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal NFIC/CTF antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NFIC aa 200-250.

View Alternative Names

NFI, NFIC, Nuclear factor 1 C-type, NF1-C, Nuclear factor 1/C, CCAAT-box-binding transcription factor, Nuclear factor I/C, TGGCA-binding protein, CTF, NF-I/C, NFI-C

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (AB245597)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (AB245597)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining. Counterstained using hematoxylin (blue).

Supplier Data

Immunoprecipitation - Anti-NFIC/CTF antibody (AB245597)

NFIC/CTF was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245597 used for IP at 6 μg/mg lysate. For WB 1 μg/ml.

Lane 1 : ab245597 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 3 seconds.

All lanes:

Immunoprecipitation - Anti-NFIC/CTF antibody (ab245597)

Predicted band size: 56 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (AB245597)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-NFIC/CTF antibody (AB245597)

All lanes:

Western blot - Anti-NFIC/CTF antibody (ab245597) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 56 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human NFIC aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P08651

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245597 was affinity purified using an epitope specific to NFIC/CTF immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

See full target information NFIC

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 19:4103-4120 PubMed38736658

2024

Long-Term Accumulation, Biological Effects and Toxicity of BSA-Coated Gold Nanoparticles in the Mouse Liver, Spleen, and Kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Jakic,Michal Selc,Filip Razga,Veronika Nemethova,Petra Mazancova,Filip Havel,Michal Sramek,Monika Zarska,Jan Proska,Vlasta Masanova,Iveta Uhnakova,Peter Makovicky,Marta Novotova,Vit Vykoukal,Andrea Babelova

eLife 11: PubMed35550030

2022

CREB5 reprograms FOXA1 nuclear interactions to promote resistance to androgen receptor-targeting therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Justin H Hwang,Rand Arafeh,Ji-Heui Seo,Sylvan C Baca,Megan Ludwig,Taylor E Arnoff,Lydia Sawyer,Camden Richter,Sydney Tape,Hannah E Bergom,Sean McSweeney,Jonathan P Rennhack,Sarah A Klingenberg,Alexander T M Cheung,Jason Kwon,Jonathan So,Steven Kregel,Eliezer M Van Allen,Justin M Drake,Matthew L Freedman,William C Hahn

Frontiers in genetics 11:846 PubMed32922434

2020

ECRG4 Represses Cell Proliferation and Invasiveness via NFIC/OGN/NF-κB Signaling Pathway in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liang,Jiangang Gao,Quan Wang,Sichuan Hou,Changli Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFIC/CTF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (AB245597)

Immunoprecipitation - Anti-NFIC/CTF antibody (AB245597)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (AB245597)

Western blot - Anti-NFIC/CTF antibody (AB245597)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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