AB93785

Anti-NFIL3 antibody

Name: Anti-NFIL3 antibody (ab93785) | Abcam
Brand: Abcam Limited
SKU: AB93785
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal NFIL3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NFIL3 aa 400 to C-terminus.

View Alternative Names

E4BP4, IL3BP1, NFIL3, Nuclear factor interleukin-3-regulated protein, E4 promoter-binding protein 4, Interleukin-3 promoter transcriptional activator, Interleukin-3-binding protein 1, Transcriptional activator NF-IL3A

2 Images

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Immunoprecipitation - Anti-NFIL3 antibody (AB93785)

Detection of NFIL3 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93785 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NFIL3 antibody (ab93785)

Predicted band size: 51 kDa

false

Unknown

Western blot - Anti-NFIL3 antibody (AB93785)

All lanes:

Western blot - Anti-NFIL3 antibody (ab93785) at 0.4 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Predicted band size: 51 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NFIL3 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16649

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death (By similarity). Represses the transcription of CYP2A5 (By similarity). Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2 (By similarity). Required for the development of natural killer cell precursors (By similarity).

See full target information NFIL3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107626 PubMed37731616

2023

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingying Zhou,Huanyu Wang,Ting Shu,Jing Wang,Weiqin Yang,Jingqing Li,Lipeng Ding,Man Liu,Hanyong Sun,John Wong,Paul Bo-San Lai,Shun-Wa Tsang,Simon E Ward,King-Lau Chow,Joseph Jao-Yiu Sung,Alfred Sze-Lok Cheng

The Journal of biological chemistry 293:5624-5635 PubMed29467226

2018

E4BP4/NFIL3 modulates the epigenetically repressed RAS effector RASSF8 function through histone methyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Isai Pratha Karthik,Pavitra Desai,Sudarkodi Sukumar,Aleksandra Dimitrijevic,Krishnaraj Rajalingam,Sundarasamy Mahalingam

PloS one 9:e106246 PubMed25162225

2014

Conversion of adipose-derived stem cells into natural killer-like cells with anti-tumor activities in nude mice.

Applications

Species

Human

Hongxiu Ning,Hong-En Lei,Yong-De Xu,Rui-Li Guan,Jeffrey M Venstrom,Guiting Lin,Tom F Lue,Zhongcheng Xin,Ching-Shwun Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFIL3 antibody

Immunoprecipitation - Anti-NFIL3 antibody (AB93785)

Western blot - Anti-NFIL3 antibody (AB93785)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise