Anti-NFIL3 antibody [EPR27211-70]

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-NFIL3 antibody [EPR27211-70] (AB309508)

Chromatin was prepared from HepG2 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab309508 [EPR27211-70]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-NFIL3 antibody [EPR27211-70] (AB309508)

Chromatin was prepared from HepG2 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab309508 [EPR27211-70]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-NFIL3 antibody [EPR27211-70] (AB309508)

Chromatin was prepared from HepG2 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab309508 [EPR27211-70]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• IP

Supplier Data

Immunoprecipitation - Anti-NFIL3 antibody [EPR27211-70] (AB309508)

NFIL3 was immunoprecipitated from 0.35mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab309508 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309508 at 1/1000 dilution (0.508 μg/ml). VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (10 μg) Lane 2 : ab309508 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309508 in HepG2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 93 seconds. Observed band : 60 kDa Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-NFIL3 antibody [EPR27211-70] (ab309508) at 1/30 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 51 kDa

Observed band size: 60 kDa

false

Exposure time: 93s

• IP

Supplier Data

Immunoprecipitation - Anti-NFIL3 antibody [EPR27211-70] (AB309508)

NFIL3 was immunoprecipitated from 0.35 mg Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab309508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309508 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 2 : ab309508 IP in Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309508 in Huh7 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 102 second. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-NFIL3 antibody [EPR27211-70] (ab309508) at 1/30 dilution

All lanes:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 102s

• IP

Supplier Data

Immunoprecipitation - Anti-NFIL3 antibody [EPR27211-70] (AB309508)

NFIL3 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab309508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309508 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 2 : ab309508 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab309508 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 171 second.

All lanes:

Immunoprecipitation - Anti-NFIL3 antibody [EPR27211-70] (ab309508) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 171s

• WB

Supplier Data

Western blot - Anti-NFIL3 antibody [EPR27211-70] (AB309508)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : JEG-3 (PMID : 29215740). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200,000 dilution. Exposure time : Lanes 1-2 : 70 seconds; Lanes 3-4 : 180 seconds; Lane 5 : 70 seconds.

All lanes:

Western blot - Anti-NFIL3 antibody [EPR27211-70] (ab309508) at 1/1000 dilution

Lane 1:

Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

JEG-3 (human placenta epithelial cell) whole cell lysate at 20 µg

Lane 3:

No-GFP-CD16.NK-92 (natural killer (nk-92®) cell lacki gfp-cd16) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa

false