Anti-NFIX Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX Antibody (AB327186)

Immunohistochemical staining of human cerebellum using ab327186 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in cells in granular layer.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX Antibody (AB327186)

Immunohistochemical staining of human prostate using ab327186 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX Antibody (AB327186)

Immunohistochemical staining of human skeletal muscle using ab327186 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in myocytes.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NFIX Antibody (AB327186)

Immunofluorescent staining of human cell line U-251 MG using ab327186 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX Antibody (AB327186)

Immunohistochemical staining of human skin using ab327186 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in squamous epithelial cells.

• ChIP

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ChIP - Anti-NFIX Antibody (AB327186)

ChIP-Exo-Seq composite graph for ab327186 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

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Western blot - Anti-NFIX Antibody (AB327186)

Western blot analysis in human cell line U-138MG using ab327186 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-NFIX Antibody (ab327186)

All lanes:

Human cell line U-138MG

Predicted band size: 55.1 kDa

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