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AB313840

Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free

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Rabbit Recombinant Monoclonal NFIX/CTF antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Rat, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human NFIX.

View Alternative Names

Nuclear factor 1 X-type, NF1-X, Nuclear factor 1/X, CCAAT-box-binding transcription factor, Nuclear factor I/X, TGGCA-binding protein, CTF, NF-I/X, NFI-X, Nfix

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum and stomach tissue labeling NFIX/CTF using ab313840 at a 1/100 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NFIX/CTF using ab313840 at a 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling NFIX/CTF using ab313840 at a 1/100 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Immunocytochemistry/ Immunofluorescence - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)

Immunofluorescent analysis of DIV10 rat E18 primary hippocampal neurons and glia labeling NFIX/CTF using ab313840 at a 1/250 dilution (green). Red : Tau, a cytoskeleton marker, stained by Tau antibody [GT287] diluted at 1/500. Cells were fixed in 4% paraformaldehyde at RT for 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIX/CTF antibody [HL1269] - BSA and Azide free (AB313840)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling NFIX/CTF using ab313840 at a 1/100 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1269

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NFIX.

Q14938

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.
See full target information Nfix

Additional targets

NFIX/CTF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com