AB203568

Anti-NFkB Inducing Kinase NIK antibody [3H31L48]

Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal NFkB Inducing Kinase NIK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K14 aa 700-900.

View Alternative Names

NIK, MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK, HsNIK

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (AB203568)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (AB203568)

Immunohistochemical analysis of non-transfected (left) and NIK transfected 293 cells (right) labeling NIK using ab203568 at 5 μg/mL. Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 dilution was used as secondary antibody (green). Hoescht staining of nucleus (blue).

Supplier Data

Western blot - Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (AB203568)

Lane 1:

anti FLAG tag antibody

Lane 2:

Western blot - Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (ab203568) at 15 µg/mL

All lanes:

Flag tagged NIK transfected 293 cell lysate

Predicted band size: 104 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

3H31L48

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MAP3K14 aa 700-900. The exact immunogen used to generate this antibody is proprietary information.

Q99558

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10-20 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "4-6 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed : 25406581, PubMed : 29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed : 25406581). Could act in a receptor-selective manner.

See full target information MAP3K14

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 8:6 PubMed36759627

2023

BMP-2 functional polypeptides relieve osteolysis via bi-regulating bone formation and resorption coupled with macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqian Wang,Yuan Xue,Yi Wang,Chang Liu,Sihan Hu,Huan Zhao,Qiaoli Gu,Huilin Yang,Lixin Huang,Xichao Zhou,Qin Shi

PLoS pathogens 18:e1010110 PubMed35797416

2022

Recruitment of the CoREST transcription repressor complexes by Nerve Growth factor IB-like receptor (Nurr1/NR4A2) mediates silencing of HIV in microglial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fengchun Ye,David Alvarez-Carbonell,Kien Nguyen,Konstantin Leskov,Yoelvis Garcia-Mesa,Sheetal Sreeram,Saba Valadkhan,Jonathan Karn

BioMed research international 2020:1481572 PubMed32908868

2020

HuR Affects Proliferation and Apoptosis of Chronic Lymphocytic Leukemia Cells via NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Xiao,Lin Yang,Xinfeng Gao,Ying An,Wei Xie,Guo Jingquan

International journal of molecular medicine 38:1661-1672 PubMed27840967

2016

Bergapten exerts inhibitory effects on diabetes-related osteoporosis via the regulation of the PI3K/AKT, JNK/MAPK and NF-κB signaling pathways in osteoprotegerin knockout mice.

Applications

Species

Mouse

Xue-Ju Li,Zhe Zhu,Si-Lin Han,Zi-Long Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFkB Inducing Kinase NIK antibody [3H31L48]

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (AB203568)

Western blot - Anti-NFkB Inducing Kinase NIK antibody [3H31L48] (AB203568)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise