Rabbit Polyclonal NFkB Inducing Kinase NIK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus.
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- Frontiers in immunology 14:11798272023LCL161 enhances expansion and survival of engineered anti-tumor T cells but is restricted by death signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArya Afsahi,Christopher M Silvestri,Allyson E Moore,Carly F Graham,Kaylyn Bacchiochi,Martine St-Jean,Christopher L Baker,Robert G Korneluk,Shawn T Beug,Eric C LaCasse,Jonathan L BramsonPubMed 37138866
- Brazilian journal of medical and biological resear 53:e92712020lncRNA PCGEM1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenxing Xu et. al.PubMed 32520202
- Nature communications 10:22472019ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaodi Fang et. al.PubMed 31113953
- Oncology letters 15:4137-41422018Tunicamycin suppresses breast cancer cell growth and metastasis via regulation of the protein kinase B/nuclear factor-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Wang et. al.PubMed 29541178
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39:10104283177055742017Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-β1-meidated ERK/JNK/NF-κB signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenzhi Yu,Lei Han,Xin Ni,Minghuan Wang,Ping Xue,Li Zhang,Mei YuanPubMed 28631556
- Current biology : CB 26:3288-33022016NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion.
- Oncogenesis 5:e2312016NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-κB pathway.
- Nature cell biology 17:1327-382015Non-canonical NF-κB signalling and ETS1/2 cooperatively drive C250T mutant TERT promoter activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinghui Li et. al.PubMed 26389665
- Reproductive sciences (Thousand Oaks, Calif.) 22:1618-262015Knockdown of Eag1 Expression by RNA Interference Increases Chemosensitivity to Cisplatin in Ovarian Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Hui et. al.PubMed 26079730
- Cell death and differentiation 22:2020-332015NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL Boutaffala et. al.PubMed 26045047
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99558
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
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Target data
Function
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed:25406581, PubMed:29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed:25406581). Could act in a receptor-selective manner.
Alternative names
NIK, MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK, HsNIK
Recommended products
Rabbit Polyclonal NFkB Inducing Kinase NIK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99558
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.
All lanes: Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
Lane 1: macrophages lysate
Lane 2: NIK transfected cell lysate
Predicted band size: 104 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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