AB7204

Anti-NFkB Inducing Kinase NIK antibody

Name: Anti-NFkB Inducing Kinase NIK antibody (ab7204) | Abcam
Brand: Abcam Limited
SKU: AB7204
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(14 Publications)

Rabbit Polyclonal NFkB Inducing Kinase NIK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus.

View Alternative Names

NIK, MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK, HsNIK

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.

All lanes:

Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)

Lane 1:

macrophages lysate

Lane 2:

NIK transfected cell lysate

Predicted band size: 104 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99558

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed : 25406581, PubMed : 29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed : 25406581). Could act in a receptor-selective manner.

See full target information MAP3K14

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1179827 PubMed37138866

2023

LCL161 enhances expansion and survival of engineered anti-tumor T cells but is restricted by death signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Arya Afsahi,Christopher M Silvestri,Allyson E Moore,Carly F Graham,Kaylyn Bacchiochi,Martine St-Jean,Christopher L Baker,Robert G Korneluk,Shawn T Beug,Eric C LaCasse,Jonathan L Bramson

Brazilian journal of medical and biological resear 53:e9271 PubMed32520202

2020

lncRNA PCGEM1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxing Xu,Lingling Meng,Yuejuan Xie,Wei Guo

Nature communications 10:2247 PubMed31113953

2019

ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Caodi Fang,Thomas D Manes,Lufang Liu,Kevin Liu,Lingfeng Qin,Guangxin Li,Zuzana Tobiasova,Nancy C Kirkiles-Smith,Manal Patel,Jonathan Merola,Whitney Fu,Rebecca Liu,Catherine Xie,Gregory T Tietjen,Peter A Nigrovic,George Tellides,Jordan S Pober,Dan Jane-Wit

Oncology letters 15:4137-4142 PubMed29541178

2018

Tunicamycin suppresses breast cancer cell growth and metastasis via regulation of the protein kinase B/nuclear factor-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Wang,Wei Xiong,Yiyin Tang

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39:1010428317705574 PubMed28631556

2017

Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-β1-meidated ERK/JNK/NF-κB signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Renzhi Yu,Lei Han,Xin Ni,Minghuan Wang,Ping Xue,Li Zhang,Mei Yuan

Current biology : CB 26:3288-3302 PubMed27889261

2016

NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion.

Applications

ICC/IF

Species

Human

Ji-Ung Jung,Sowndharya Ravi,Dong W Lee,Kassandra McFadden,Michael L Kamradt,L Gerard Toussaint,Raquel Sitcheran

Oncogenesis 5:e231 PubMed27270613

2016

NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-κB pathway.

Applications

IHC

Species

Unspecified reactive species

C L Duran,D W Lee,J-U Jung,S Ravi,C B Pogue,L G Toussaint,K J Bayless,R Sitcheran

Nature cell biology 17:1327-38 PubMed26389665

2015

Non-canonical NF-κB signalling and ETS1/2 cooperatively drive C250T mutant TERT promoter activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Li,Qi-Ling Zhou,Wenjie Sun,Prashant Chandrasekharan,Hui Shan Cheng,Zhe Ying,Manikandan Lakshmanan,Anandhkumar Raju,Daniel G Tenen,Shi-Yuan Cheng,Kai-Hsiang Chuang,Jun Li,Shyam Prabhakar,Mengfeng Li,Vinay Tergaonkar

Reproductive sciences (Thousand Oaks, Calif.) 22:1618-26 PubMed26079730

2015

Knockdown of Eag1 Expression by RNA Interference Increases Chemosensitivity to Cisplatin in Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Hui,Zhang Lan,Lin Yue-li,Hong Li-lin,Huang Li-lin

Cell death and differentiation 22:2020-33 PubMed26045047

2015

NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

L Boutaffala,M J M Bertrand,C Remouchamps,G Seleznik,F Reisinger,M Janas,C Bénézech,M T Fernandes,S Marchetti,F Mair,C Ganeff,A Hupalowska,J-E Ricci,B Becher,J Piette,P Knolle,J Caamano,P Vandenabeele,M Heikenwalder,E Dejardin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFkB Inducing Kinase NIK antibody

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

Western blot - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Product promise