Rabbit Recombinant Monoclonal NFkB Inducing Kinase NIK antibody. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.
Images
![Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146), expandable thumbnail](https://content.abcam.com/products/images/nfkb-inducing-kinase-nik-antibody-epr26101-23-ab314146--western-blot-img419354.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146)")
![Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146), expandable thumbnail](https://content.abcam.com/products/images/nfkb-inducing-kinase-nik-antibody-epr26101-23-ab314146--western-blot-img419355.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146)")
![Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146), expandable thumbnail](https://content.abcam.com/products/images/nfkb-inducing-kinase-nik-antibody-epr26101-23-ab314146--western-blot-img419356.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed:25406581, PubMed:29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed:25406581). Could act in a receptor-selective manner.
Alternative names
NIK, MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK, HsNIK
Recommended products
Rabbit Recombinant Monoclonal NFkB Inducing Kinase NIK antibody. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26101-23
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146), expandable thumbnail](https://content.abcam.com/products/images/nfkb-inducing-kinase-nik-antibody-epr26101-23-ab314146--western-blot-img419354.jpg "Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146)")
Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of NFkB Inducing Kinase NIK is upregulated in response to MG132 treatment (PMID:24600451, PMID: 25792747, PMID: 18305221).
The identity of the lower MW band at approximately 37 kDa is unknown.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146) at 1/1000 dilution
Lane 1: Untreated MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MCF7 treated with 10 uM MG132 for 2 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 125 kDa
Exposure time: 180s
![Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146), expandable thumbnail](https://content.abcam.com/products/images/nfkb-inducing-kinase-nik-antibody-epr26101-23-ab314146--western-blot-img419355.jpg "Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146)")
Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: colon (PMID: 9020361).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24600451, 24656046).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The lanes 4-6 were developed using a high sensitivity ECL substrate.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Mouse colon tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat spleen tissue lysate at 20 µg
Lane 6: Rat colon tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 125 kDa
Exposure time: 180s
![Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146), expandable thumbnail](https://content.abcam.com/products/images/nfkb-inducing-kinase-nik-antibody-epr26101-23-ab314146--western-blot-img419356.jpg "Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146)")
Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 26 secondsAll lanes: Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human MAP3K14 expression vector containi a His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 125 kDa
Exposure time: 26s
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