Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23]

• WB

Supplier Data

Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of NFkB Inducing Kinase NIK is upregulated in response to MG132 treatment (PMID : 24600451, PMID : 25792747, PMID : 18305221).

The identity of the lower MW band at approximately 37 kDa is unknown.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146) at 1/1000 dilution

Lane 1:

Untreated MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 treated with 10 uM MG132 for 2 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : colon (PMID : 9020361). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24600451, 24656046). Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The lanes 4-6 were developed using a high sensitivity ECL substrate. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse colon tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Lane 6:

Rat colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (AB314146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 26 seconds

All lanes:

Western blot - Anti-NFkB Inducing Kinase NIK antibody [EPR26101-23] (ab314146) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human MAP3K14 expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa

false

Exposure time: 26s