AB209799

Anti-NFkB p100/NFKB2 antibody

Name: Anti-NFkB p100/NFKB2 antibody (ab209799) | Abcam
Brand: Abcam Limited
SKU: AB209799

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(1 Publication)

Rabbit Polyclonal NFkB p100/NFKB2 phospho S865 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NFKB2 phospho S865 aa 700 to C-terminus.

View Alternative Names

LYT10, NFKB2, Nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, Lyt10

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human appendix tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (AB209799)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling NFkB p100/NFKB2 using ab209799 at 4 μg/ml (green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-NFkB p100/NFKB2 antibody (AB209799)

Loading control : Anti-GAPDH

All lanes:

Western blot - Anti-NFkB p100/NFKB2 antibody (ab209799) at 0.4 µg/mL

Lane 1:

A431 (human epidermoid carcinoma cell line) transfected with control siRNA, cell lysate

Lane 2:

A431 transfected with target specific siRNA probe #1, cell lysate

Lane 3:

A431 transfected with target specific siRNA probe #2, cell lysate

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NFKB2 phospho S865 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q00653

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.

See full target information NFKB2 phospho S865

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:863 PubMed36220838

2022

Neddylation-mediated degradation of hnRNPA2B1 contributes to hypertriglyceridemia pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Chen,Yilong Wang,Wenwen Xia,Jinbao Zhang,Yan Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFkB p100/NFKB2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (AB209799)

Western blot - Anti-NFkB p100/NFKB2 antibody (AB209799)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise