Name: Anti-NFkB p105 / p50 antibody [E381]
SKU: ab32360
Rating: 4 (21 reviews)

Anti-NFkB p105 / p50 antibody [E381] (ab32360) is a rabbit monoclonal antibody that is used to detect NFkB p105 / p50 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with NFkB p105 / p50 knockout cell line validation

Related conjugates and formulations

ab195854
Conjugated
HRP Anti-NFkB p105 / p50 antibody [E381]
ab220803
Carrier free
Anti-NFkB p105 / p50 antibody [E381] - BSA and Azide free
ab313112
Conjugated
Alexa Fluor® 568 Anti-NFkB p105 / p50 antibody [E381]
ab313305
Conjugated
Alexa Fluor® 555 Anti-NFkB p105 / p50 antibody [E381]
ab311829
Conjugated
Alexa Fluor® 594 Anti-NFkB p105 / p50 antibody [E381]
ab311174
Conjugated
Alexa Fluor® 647 Anti-NFkB p105 / p50 antibody [E381]
ab321590
Conjugated
Alexa Fluor® 750 Anti-NFkB p105 / p50 antibody [E381]

Images

Western blot - Anti-NFkB p105 / p50 antibody [E381] (AB32360), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	Flow Cyt	IHC-P	IP
Human	Tested	Not recommended	Tested	Not recommended
Mouse	Expected	Not recommended	Predicted	Not recommended
Rat	Tested	Not recommended	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000 - 1/10000	Notes -
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Species Human	Dilution info -	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information NFKB1

Function

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. Nuclear factor NF-kappa-B p105 subunit. P105 is the precursor of the active p50 subunit (Nuclear factor NF-kappa-B p50 subunit) of the nuclear factor NF-kappa-B (PubMed:1423592). Acts as a cytoplasmic retention of attached NF-kappa-B proteins by p105 (PubMed:1423592). Nuclear factor NF-kappa-B p50 subunit. Constitutes the active form, which associates with RELA/p65 to form the NF-kappa-B p65-p50 complex to form a transcription factor (PubMed:1740106, PubMed:7830764). Together with RELA/p65, binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions (PubMed:1740106, PubMed:7830764).

Alternative names

Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, NFKB1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: E381
Purification technique: Affinity purification Protein A
Specificity: This antibody will detect both forms: p50 and p105.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-NFkB p105 / p50 antibody [E381] (ab32360) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of NFkB p105 / p50?

Anti-NFkB p105 / p50 [E381] (ab32360) specifically detects a band for NFkB p105 / p50 (UniProt: P19838) at a molecular weight of 50kDa.

Trusted by the scientific community

Anti-NFkB p105 / p50 [E381] (ab32360) was first used in a scientific publication in 2006 and has been cited over 210 times in peer-reviewed journals.

Reviewed by scientists

Anti-NFkB p105 / p50 [E381] (ab32360) has over 20 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-NFkB p105 / p50 antibody [E381] (ab32360) has been confirmed by Western blot testing in NFKB1 Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [E381] also available for your convenience:
ab32360, HRP - ab195854, Carrier free - ab220803, Alexa Fluor® 647 - ab311174, Alexa Fluor® 594 - ab311829, Alexa Fluor® 568 - ab313112, Alexa Fluor® 555 - ab313305, Alexa Fluor® 750 - ab321590

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Lanes 1 - 4: Merged signal (red and green). Green - ab32360 observed at 120, 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab32360 was shown to specifically react with NFkB p105 / p50 when NFkB p105 / p50 knockout samples were used. Wild-type and NFkB p105 / p50 knockout samples were subjected to SDS-PAGE. ab32360 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: NFkB p105 / p50 knockout HAP1 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
Predicted band size: 105 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue labelling NFkB p105 / p50 with purified ab32360 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/10000 dilution
All lanes: HeLa cell lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa, 50 kDa
Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Lanes 1- 2: Merged signal (red and green). Green - ab32360 observed at 105 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab32360 was shown to react with NFkB p105 / p50 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264823 (CRISPR/Cas9 edited cell lysate ab257003) lane below 105kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and NFKB1 CRISPR/Cas9 HeLa HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32360 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: NFKB1 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/50000 dilution
All lanes: MCF-7 cell lysate at 10 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa, 50 kDa
Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/10000 dilution
Lane 1: PC-12 cell lysate at 20 µg
Lane 2: NIH/3T3 cell lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa, 50 kDa
Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
All lanes: Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/5000 dilution
All lanes: HeLa cell lysate
Predicted band size: 105 kDa
Observed band size: 105 kDa, 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NFkB p105 / p50 with unpurified ab32360 at a dilution of 1/250.
This image is courtesy of an anonymous customer review
Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360)
All lanes: Western blot - Anti-NFkB p105 / p50 antibody [E381] (ab32360) at 1/1000 dilution
All lanes: mouse neural stem cell lysate at 40 µg
Secondary
All lanes: Alexa Fluor 790 Goat fluorescent secondary at 1/10000 dilution
Performed under reducing conditions.
Exposure time: 1hr