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Rabbit Multiclonal NFkB p105 / p50 phospho S337 antibody. Suitable for WB, ChIP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NFKB1 phospho S337.

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Images

ChIP - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (AB308112), expandable thumbnail
  • Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (AB308112), expandable thumbnail
  • Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (AB308112), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Synthetic Peptide within Human NFKB1 phospho S337. Database link P19838

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBChIP
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.Nuclear factor NF-kappa-B p105 subunitP105 is the precursor of the active p50 subunit (Nuclear factor NF-kappa-B p50 subunit) of the nuclear factor NF-kappa-B (PubMed:1423592). Acts as a cytoplasmic retention of attached NF-kappa-B proteins by p105 (PubMed:1423592).Nuclear factor NF-kappa-B p50 subunitConstitutes the active form, which associates with RELA/p65 to form the NF-kappa-B p65-p50 complex to form a transcription factor (PubMed:1740106, PubMed:7830764). Together with RELA/p65, binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions (PubMed:1740106, PubMed:7830764).

Alternative names

Recommended products

Rabbit Multiclonal NFkB p105 / p50 phospho S337 antibody. Suitable for WB, ChIP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NFKB1 phospho S337.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Synthetic Peptide within Human NFKB1 phospho S337. Database link P19838
Clone number

RP23040087

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

3 product images

  • ChIP - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112), expandable thumbnail

    ChIP - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112)

    Enrichment of endogenous NFkB p50 pSer337 protein at specific gene loci using ab308112: Chromatin Immunoprecipitation (ChIP) was performed using ab308112, 5 μg on sheared chromatin from 2 million HeLa cells treated with 50 ng/mL of TNFalpha for 45 minutes. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed with optimized PCR primer pairs for the promoter of active IL-8, IkB gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

  • Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112), expandable thumbnail

    Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112)

    Western blot analysis of NFkB p105 / p50 (phospho S337) in whole cell extracts from TNFa treated Jurkat (20 ng/mL, 15 min) using ab308112 at a dilution of 1 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~50 kDa.

    All lanes: Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112) at 1 µg/mL

    All lanes: whole cell extracts from TNFa treated Jurkat cells

    Secondary

    All lanes: HRP-conjugated Goat anti-Rabbit secondary antibody

    Developed using the ECL technique.

    Observed band size: 50 kDa

  • Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112), expandable thumbnail

    Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112)

    Western blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with TNF alpha (Lane 2). The blots were probed with ab308112, 1-2 µg/mL and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to NFkB p105 / p50 (phospho S337) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-NFkB p105 / p50 (phospho S337) antibody [RP23040087] - ChIP Grade (ab308112) at 1 µg/mL

    Lane 1: Jurkat whole cell extract at 30 µg

    Lane 2: Jurkat treated with TNF alpha at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 105 kDa

    Observed band size: 55 kDa

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Product protocols

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