AB101898

Anti-NFKBIL2 antibody

Name: Anti-NFKBIL2 antibody (ab101898) | Abcam
Brand: Abcam Limited
SKU: AB101898
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal NFKBIL2 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

IKBR, NFKBIL2, TONSL, Tonsoku-like protein, Inhibitor of kappa B-related protein, NF-kappa-B inhibitor-like protein 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 2, I-kappa-B-related protein, IkappaBR

1 Images

Unknown

Western blot - Anti-NFKBIL2 antibody (AB101898)

ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID : 21055983).

All lanes:

Western blot - Anti-NFKBIL2 antibody (ab101898) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 151 kDa

Observed band size: 175 kDa,43 kDa,67 kDa,80 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Leaf frog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the MMS22L-TONSL complex, a complex that promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 21113133, PubMed : 26527279, PubMed : 27338793, PubMed : 27797818, PubMed : 29478807, PubMed : 30773278). The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985). It mediates the assembly of RAD51 filaments on single-stranded DNA (ssDNA) : the MMS22L-TONSL complex is recruited to DSBs following histone replacement by histone chaperones and eviction of the replication protein A complex (RPA/RP-A) from DSBs (PubMed : 21055983, PubMed : 21055984, PubMed : 21055985, PubMed : 27797818, PubMed : 29478807). Following recruitment to DSBs, the TONSL-MMS22L complex promotes recruitment of RAD51 filaments and subsequent homologous recombination (PubMed : 27797818, PubMed : 29478807). Within the complex, TONSL acts as a histone reader, which recognizes and binds newly synthesized histones following their replacement by histone chaperones (PubMed : 27338793, PubMed : 29478807). Specifically binds histone H4 lacking methylation at 'Lys-20' (H4K20me0) and histone H3.1 (PubMed : 27338793).

See full target information TONSL

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298484

2023

Oncogenic Impact of TONSL, a Homologous Recombination Repair Protein at the Replication Fork, in Cancer Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hani Lee,Sojung Ha,SeokGyeong Choi,Soomin Do,Sukjoon Yoon,Yong Kee Kim,Woo-Young Kim

Journal of cellular biochemistry 119:4692-4710 PubMed29278662

2018

MicroRNA-335-5p suppresses lower extremity deep venous thrombosis by targeted inhibition of PAI-1 via the TLR4 signalingpathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Xia Bao,Dong-Xia Zhang,Na-Na Wang,Xiang-Kui Zhu,Qi Zhao,Xiao-Lei Sun

Molecular cell 69:879-892.e5 PubMed29478807

2018

The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Hsiang Huang,Faith Fowler,Chin-Chuan Chen,Zih-Jie Shen,Barry Sleckman,Jessica K Tyler

Nature 534:714-718 PubMed27338793

2016

H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex.

Applications

Species

Unspecified reactive species

Giulia Saredi,Hongda Huang,Colin M Hammond,Constance Alabert,Simon Bekker-Jensen,Ignasi Forne,Nazaret Reverón-Gómez,Benjamin M Foster,Lucie Mlejnkova,Till Bartke,Petr Cejka,Niels Mailand,Axel Imhof,Dinshaw J Patel,Anja Groth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFKBIL2 antibody

Western blot - Anti-NFKBIL2 antibody (AB101898)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise