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AB229829

Anti-NFS1 antibody

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(3 Publications)

Rabbit Polyclonal NFS1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFS1 aa 200 to C-terminus.

View Alternative Names

NIFS, HUSSY-08, NFS1, Cysteine desulfurase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFS1 antibody (AB229829)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFS1 antibody (AB229829)

Paraffin-embedded human colon cancer tissue stained for NFS1 using ab229829 at 1/100 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-NFS1 antibody (AB229829)
  • IP

Supplier Data

Immunoprecipitation - Anti-NFS1 antibody (AB229829)

NFS1 was immunoprecipitated from 500 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg ab229829. An HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as secondary antibody at 1/50000 dilution.

Lane 1 : Rabbit monoclonal IgG IP in HeLa whole cell lysate.

Lane 2 : ab229829 IP in HeLa whole cell extract.

Lane 3 : HeLa whole cell lysate 20 μg (Input).

All lanes:

Immunoprecipitation - Anti-NFS1 antibody (ab229829)

Predicted band size: 50 kDa

false

Western blot - Anti-NFS1 antibody (AB229829)
  • WB

Supplier Data

Western blot - Anti-NFS1 antibody (AB229829)

All lanes:

Western blot - Anti-NFS1 antibody (ab229829) at 1/1000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

Mouse kidney lysate

Lane 4:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NFS1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y697

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform Mitochondrial. Cysteine desulfurase, of the core iron-sulfur cluster (ISC) assembly complex, that catalyzes the desulfuration of L-cysteine to L-alanine, as component of the cysteine desulfurase complex, leading to the formation of a cysteine persulfide intermediate at the active site cysteine residue and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed : 18650437, PubMed : 29097656, PubMed : 31101807). The persulfide is then transferred on the flexible Cys loop from the catalytic site of NFS1 to the surface of NFS1 (PubMed : 29097656). After the NFS1-linked persulfide sulfur is transferred to one of the conserved Cys residues of the scaffold, a reaction assisted by FXN (By similarity). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity).. Isoform Cytoplasmic. May catalyze the desulfuration of L-cysteine to L-alanine as component of the cysteine desulfurase complex (NFS1 : LYRM4), leading to the formation of a cysteine persulfide intermediate (PubMed : 16527810, PubMed : 18650437). Acts as a sulfur donor for MOCS3 by transferring the sulfur of the cysteine persulfide intermediate on MOCS3 (PubMed : 18650437, PubMed : 23593335).
See full target information NFS1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110234 PubMed40378953

2025

Antioxidant capacity of the iron-sulfur cluster assembly protein IscU2 is mediated by aspartate metabolism to promote tumor survival.

Applications

Unspecified application

Species

Unspecified reactive species

Xunjun Yang,Na Liang,Dandan Liu,Jimei Yan,Xiali Yang,Jinya Lv,Saijun Xiao,Xiujuan Wei,Xuyang Chen,Zhengquan Yang,Shanying Gui,Liqin Jin,Shihui Yu,Jianxin Lyu,Xiaojun Ren

eLife 10: PubMed34612205

2021

Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites.

Applications

Unspecified application

Species

Unspecified reactive species

Seyi Falekun,Jaime Sepulveda,Yasaman Jami-Alahmadi,Hahnbeom Park,James A Wohlschlegel,Paul A Sigala

Neoplasma 66:564-575 PubMed30943745

2019

LncRNA TTN-AS1 contributes to gastric cancer progression by acting as a competing endogenous RNA of miR-376b-3p.

Applications

Unspecified application

Species

Unspecified reactive species

M M Dong,S J Peng,Y N Yuan,H P Luo
View all publications
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