Rabbit Polyclonal NFX1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NFX1 aa 1050 to C-terminus.
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Publications
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- Cancer immunology research :2020Tumor cell-derived TGFβ1 Attenuates Antitumor Immune Activity of T cells via Regulation of PD-1 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengfei Wu et. al.PubMed 32999004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NFX1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12986
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Target data
Function
Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.
Alternative names
NFX2, NFX1, Transcriptional repressor NF-X1
Recommended products
Rabbit Polyclonal NFX1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NFX1 aa 1050 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NFX1 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q12986
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab176733 is affinity purified using an epitope specific to NFX1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-NFX1 antibody (ab176733)
All lanes: Western blot - Anti-NFX1 antibody (ab176733) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30s
Immunoprecipitation - Anti-NFX1 antibody (ab176733)
ab176733 at 0.4 μg/ml detecting NFX1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of NFX1.
Lane 2: IP with an antibody which recognizes an upstream epitope of NFX1.Lane 3: ab176733 at 3 μg/mg of lysate
Lane 4: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-NFX1 antibody (ab176733)
Predicted band size: 124 kDa
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