Anti-NFX1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-NFX1 antibody (AB176733)

ab176733 at 0.4 μg/ml detecting NFX1 in HeLa whole cell lysate by WB following IP.

Lane 1 : IP with an antibody which recognizes an upstream epitope of NFX1.
Lane 2 : IP with an antibody which recognizes an upstream epitope of NFX1.

Lane 3 : ab176733 at 3 μg/mg of lysate

Lane 4 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NFX1 antibody (ab176733)

Predicted band size: 124 kDa

false

• WB

Supplier Data

Western blot - Anti-NFX1 antibody (AB176733)

All lanes:

Western blot - Anti-NFX1 antibody (ab176733) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 124 kDa

true

Exposure time: 30s