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AB154554

Anti-NFYA antibody

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(2 Publications)

Rabbit Polyclonal NFYA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFYA aa 1-350.

View Alternative Names

Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (AB154554)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (AB154554)

Immunohistochemical analysis of paraffin embedded Human D54 xenograft labeling NFYA with ab154554 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (AB154554)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody (AB154554)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling NFYA protein at nucleus using ab154554 at a 1/500 dilution (Green).
phalloidin, a cytoskeleton marker, was stained at a 1/100 dilution (Red). Scale bar = 10 µm

Western blot - Anti-NFYA antibody (AB154554)
  • WB

Supplier Data

Western blot - Anti-NFYA antibody (AB154554)

10% SDS PAGE

All lanes:

Western blot - Anti-NFYA antibody (ab154554) at 1/3000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Western blot - Anti-NFYA antibody (AB154554)
  • WB

Supplier Data

Western blot - Anti-NFYA antibody (AB154554)

10% SDS PAGE

All lanes:

Western blot - Anti-NFYA antibody (ab154554) at 1/3000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Lane 3:

BCL1 whole cell lysate at 30 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NFYA aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P23511

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component BMAL1.
See full target information NFYA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 58:2867-2880.e7 PubMed37972593

2023

Transcription factor NFYa controls cardiomyocyte metabolism and proliferation during mouse fetal heart development.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Cui,Svetlana Bezprozvannaya,Tian Hao,Abdallah Elnwasany,Luke I Szweda,Ning Liu,Rhonda Bassel-Duby,Eric N Olson

International journal of molecular sciences 24: PubMed37762002

2023

Advanced Optical Microscopy: Unveiling Functional Insights Regarding a Novel Variant and Its Unreported Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Mònica Roldán,Gregorio Alexander Nolasco,Lluís Armengol,Marcos Frías,Marta Morell,Manel García-Aragonés,Florencia Epifani,Jordi Muchart,María Luisa Ramírez-Almaraz,Loreto Martorell,Cristina Hernando-Davalillo,Roser Urreizti,Mercedes Serrano
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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