JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB264275

Anti-NFYA antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal NFYA antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NFYA aa 300 to C-terminus.

View Alternative Names

Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (AB264275)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (AB264275)

Immunohistochemical analysis of FFPE human breast carcinoma tissue section (left) and FFPE mouse hybridome tumour tissue section (right) labelling NFYA with ab264275 at 1/1000 dilution. Detection : DAB.

Immunoprecipitation - Anti-NFYA antibody (AB264275)
  • IP

Supplier Data

Immunoprecipitation - Anti-NFYA antibody (AB264275)

NFYA was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264275 at 30μg per reaction. Western blot was performed on the immunoprecipitates using ab264275 at 1 μg/ml.

Lane 1 : ab264275 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in Hela whole cell lysate.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-NFYA antibody (ab264275)

Predicted band size: 37 kDa

false

Western blot - Anti-NFYA antibody (AB264275)
  • WB

Supplier Data

Western blot - Anti-NFYA antibody (AB264275)

All lanes:

Western blot - Anti-NFYA antibody (ab264275) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Lane 5:

NIH-3T3 whole cell lysate at 50 µg

Predicted band size: 37 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human NFYA aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P23511

style
left-column

Alternative Products

Primary Antibodies

AB248874

Anti-NFYA antibody [EPR9061] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] - BSA and Azide free (AB248874)

  • 0
  • 0
Primary Antibodies

AB154554

Anti-NFYA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA antibody (AB154554)

  • 0
  • 0
Primary Antibodies

AB139402

Anti-NFYA antibody [EPR9061]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (AB139402)

  • 5
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB17721

Anti-KDM1/LSD1 antibody - Nuclear Marker

KO Validated|Lab Essentials

Western blot - Anti-KDM1/LSD1 antibody - Nuclear Marker (AB17721)

  • 4
  • 25
Primary Antibodies

AB307198

Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free

RabMAb|KO Validated

Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198)

  • 0
  • 0
Primary Antibodies

AB188453

Anti-Dnmt1 antibody [EPR18453]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Dnmt1 antibody [EPR18453] (AB188453)

  • 5
  • 7
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component BMAL1.
See full target information NFYA
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Medical science monitor : international medical journal of experimental and clinical research 29:e938410 PubMed36680333

2023

NFYAv1 is a Tumor-Promoting Transcript Associated with Poor Prognosis of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zigang Li,Chengshan You,Jun Huang,Ming Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com