JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327236

Anti-NFYA Antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal NFYA antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NFYA.

View Alternative Names

Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)

Immunohistochemical staining of human fallopian tube using ab327236 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)

Immunohistochemical staining of human tonsil using ab327236 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in non-germinal center cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)

Immunohistochemical staining of human small intestine using ab327236 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)

Immunohistochemical staining of human testis using ab327236 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in cells in seminiferous ducts.

Immunocytochemistry/ Immunofluorescence - Anti-NFYA Antibody (AB327236)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFYA Antibody (AB327236)

Immunofluorescent staining of human cell line U-2 OS using ab327236 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-NFYA Antibody (AB327236)
  • ChIP

Supplier Data

ChIP - Anti-NFYA Antibody (AB327236)

ChIP-Exo-Seq composite graph for ab327236 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-NFYA Antibody (AB327236)
  • WB

Supplier Data

Western blot - Anti-NFYA Antibody (AB327236)

Western blot analysis using ab327236 at 0.04–0.4 µg/ml.
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Human cell line RT-4
Lane 3 : Human cell line U-251MG sp

All lanes:

Western blot - Anti-NFYA Antibody (ab327236)

Lane 2:

Human cell line RT-4

Lane 3:

Human cell line U-251MG sp

Predicted band size: 36.88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NFYA.

P23511

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component BMAL1.
See full target information NFYA

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com