Rabbit Polyclonal NFYA antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NFYA.
View Alternative Names
Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
Immunohistochemical staining of human fallopian tube using ab327236 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
Immunohistochemical staining of human tonsil using ab327236 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in non-germinal center cells.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
Immunohistochemical staining of human small intestine using ab327236 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYA Antibody (AB327236)
Immunohistochemical staining of human testis using ab327236 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in cells in seminiferous ducts.
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-NFYA Antibody (AB327236)
Immunofluorescent staining of human cell line U-2 OS using ab327236 at 0.25–2 µg/ml, showing localization to nucleoplasm.
- ChIP
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ChIP - Anti-NFYA Antibody (AB327236)
ChIP-Exo-Seq composite graph for ab327236 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
- WB
Supplier Data
Western blot - Anti-NFYA Antibody (AB327236)
Western blot analysis using ab327236 at 0.04–0.4 µg/ml.
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Human cell line RT-4
Lane 3 : Human cell line U-251MG sp
All lanes:
Western blot - Anti-NFYA Antibody (ab327236)
Lane 2:
Human cell line RT-4
Lane 3:
Human cell line U-251MG sp
Predicted band size: 36.88 kDa
false
Reactivity data
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