AB139402

Anti-NFYA antibody [EPR9061]

Name: Anti-NFYA antibody [EPR9061] (ab139402) | Abcam
Brand: Abcam Limited
SKU: AB139402
Price: 395 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal NFYA antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 5 publications.

View Alternative Names

Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA, NFYA

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (AB139402)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (AB139402)

ab139402 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab139402 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Flow Cytometry (Intracellular) - Anti-NFYA antibody [EPR9061] (AB139402)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-NFYA antibody [EPR9061] (AB139402)

Intracellular flow cytometrical analysis of permeabilized NIH 3T3 cells labelling NFYA with ab139402 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-NFYA antibody [EPR9061] (AB139402)

All lanes:

Western blot - Anti-NFYA antibody [EPR9061] (ab139402) at 1/1000 dilution

Lane 1:

NIH/3T3 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9061

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component BMAL1.

See full target information NFYA

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70088 PubMed40511723

2025

Enhanced Early Detection of Colorectal Cancer via Blood Biomarker Combinations Identified Through Extracellular Vesicle Isolation and Artificial Intelligence Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Bonhan Koo,Young Il Kim,Minju Lee,Seok-Byung Lim,Yong Shin

Discover oncology 15:528 PubMed39367986

2024

NFYA-mediated promotion of castration-resistant prostate cancer progression through EGR4 regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Guijiang Sun,Yi Shao,Qianwang Ma,Shengju Song,Yutong Chen,Yang Li,Yue Gao,Haitao Wang,Zhiqun Shang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22779 PubMed36723798

2023

ANP promotes HTR-8/SVneo cell invasion by upregulating protein kinase N 3 via autophagy inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Chu,Yao Tang,Cheng-Jie Wang,Jiang-Nan Pei,Shou-Ling Luo,Yi Yu,Zhen-Zhen Liu,Hai-Yan Liu,Xue-Min Qiu,Ling Wang,Da-Jin Li,Wei-Rong Gu

Molecular cell 78:752-764.e6 PubMed32333838

2020

DNA Methylation Regulates Alternative Polyadenylation via CTCF and the Cohesin Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Vishal Nanavaty,Elizabeth W Abrash,Changjin Hong,Sunho Park,Emily E Fink,Zhuangyue Li,Thomas J Sweet,Jeffrey M Bhasin,Srinidhi Singuri,Byron H Lee,Tae Hyun Hwang,Angela H Ting

Biotechnology progress 34:1581-1588 PubMed30294957

2018

Enhancement of transgene expression by nuclear transcription factor Y and CCCTC-binding factor.

Applications

Unspecified application

Species

Unspecified reactive species

Devon Zimmerman,Krupa Patel,Matthew Hall,Jacob Elmer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFYA antibody [EPR9061]

Immunocytochemistry/ Immunofluorescence - Anti-NFYA antibody [EPR9061] (AB139402)

Flow Cytometry (Intracellular) - Anti-NFYA antibody [EPR9061] (AB139402)

Western blot - Anti-NFYA antibody [EPR9061] (AB139402)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (5)

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