AB111577

Anti-NFYB/CBF-B antibody

Name: Anti-NFYB/CBF-B antibody (ab111577) | Abcam
Brand: Abcam Limited
SKU: AB111577
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal NFYB/CBF-B antibody. Suitable for WB, ICC/IF, IHC-P, IP and reacts with Human, Mouse, Rat, Zebrafish samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NFYB aa 1-200.

View Alternative Names

HAP3, NFYB, Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFYB/CBF-B antibody (AB111577)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFYB/CBF-B antibody (AB111577)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling NFYB protein at nucleus using ab111577 at a 1/500 dilution (Green). phalloidin, a cytoskeleton marker was stained by anti-phalloidin antibody at a 1/100 dilution (Red).

Scale bar = 10 µm

Supplier Data

Immunoprecipitation - Anti-NFYB/CBF-B antibody (AB111577)

Samples were separated by10% SDS-PAGE. The immunoprecipitated NFYB protein was detected by ab111577 diluted at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-NFYB/CBF-B antibody (ab111577) at 2.5 µg

Lane 1:

293T whole cell lysate

Lane 2:

293T whole cell lysate with control IgG

Lane 3:

293T whole cell lysate (+)

Secondary

All lanes:

Anti-rabbit IgG (HRP)

Predicted band size: 23 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYB/CBF-B antibody (AB111577)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYB/CBF-B antibody (AB111577)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue stained for NFYB protein at nucleus in mouse intestine with ab111577 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYB/CBF-B antibody (AB111577)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue stained for NFYB protein at nucleus in rat stomach with ab111577 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-NFYB/CBF-B antibody (ab111577) at 1/1000 dilution

Lane 1:

HeLa whole cell extract at 30 µg

Lane 2:

HeLa nuclear extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-NFYB/CBF-B antibody (ab111577) at 1/1000 dilution

Lane 1:

PC12 whole cell extract at 30 µg

Lane 2:

Rat2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Samples were separated by 12 % SDS.

All lanes:

Western blot - Anti-NFYB/CBF-B antibody (ab111577) at 1/1000 dilution

Lane 1:

Neuro2a whole cell extract at 30 µg

Lane 2:

C8D30 whole cell extract at 30 µg

Lane 3:

NIH-3T3 whole cell extract at 30 µg

Lane 4:

Raw264.7 whole cell extract at 30 µg

Lane 5:

C2C12 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Samples were separated by 12 % SDS. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-NFYB/CBF-B antibody (ab111577) at 1/1000 dilution

Lane 1:

Zebrafish brain tissue extract at 30 µg

Lane 2:

Zebrafish eye tissue extract at 30 µg

Lane 3:

Whole zebrafish tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 23 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

ICC/IF, WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NFYB aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P25208

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.

See full target information NFYB

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0313939 PubMed39585848

2024

Validation of the biological function and prognostic significance of AURKA in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chu

Clinics (Sao Paulo, Brazil) 79:100365 PubMed38677194

2024

Tanshinone IIA alleviates IL-1β-induced chondrocyte apoptosis and inflammation by regulating FBXO11 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Xu,XiaoCheng Zhi,YunHui Zhang,Ren Ding

Medicine 102:e32885 PubMed36820591

2023

The neuroprotective effect of electro-acupuncture on cognitive recovery for patients with mild traumatic brain injury: A randomized controlled clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Haokun Jia,Yonghan Chen,Yi Wang,Linwei Jia,Yaohui Tian,Hao Jiang

Cell death & disease 13:249 PubMed35301297

2022

Skp2 stabilizes Mcl-1 and confers radioresistance in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinfang Yu,Li Zhou,Wenbin Liu,Lijun Liu,Feng Gao,Wei Li,Haidan Liu

Bioengineered 13:1459-1475 PubMed35258414

2022

Exosome-delivered BMP-2 and polyaspartic acid promotes tendon bone healing in rotator cuff tear via Smad/RUNX2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Han,Hong Liu,Huajun Fu,Yugen Hu,Weili Fang,Junsheng Liu

Cancer science 112:2770-2780 PubMed33934437

2021

CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxiao Shi,Atsuko Niimi,Toshiyuki Takeuchi,Kazuya Shiogama,Yasuyoshi Mizutani,Taisuke Kajino,Kenichi Inada,Tetsunari Hase,Takahiro Hatta,Hirofumi Shibata,Takayuki Fukui,Toyofumi Fengshi Chen-Yoshikawa,Kazuki Nagano,Takashi Murate,Yoshiyuki Kawamoto,Shuta Tomida,Takashi Takahashi,Motoshi Suzuki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFYB/CBF-B antibody

Immunocytochemistry/ Immunofluorescence - Anti-NFYB/CBF-B antibody (AB111577)

Immunoprecipitation - Anti-NFYB/CBF-B antibody (AB111577)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYB/CBF-B antibody (AB111577)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFYB/CBF-B antibody (AB111577)

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Western blot - Anti-NFYB/CBF-B antibody (AB111577)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise