Anti-NG2 antibody [132.38]

• WB

Supplier Data

Western blot - Anti-NG2 antibody [132.38] (AB50009)

All lanes:

Western blot - Anti-NG2 antibody [132.38] (ab50009) at 1 µg/mL

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat spinal cord tissue lysate at 20 µg

Lane 3:

Rat hippocampus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 251 kDa

Observed band size: 270 kDa,31 kDa,41 kDa

true

Exposure time: 20min

• WB

CiteAb

Western blot - Anti-NG2 antibody [132.38] (AB50009)

NG2 western blot using anti-NG2 antibody [132.38] ab50009. Publication image and figure legend from Li, M. Z., Zhan, Y., et al., 2020, Front Physiol, PubMed 31920724.

ab50009 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab50009 please see the product overview.

Effect of XSEC or EE and their combination on oligodendrogenesis in MCAO rats. (A) A representative coronal T2WI image at the level of bregma 0.2 mm. Boxes indicated the peri-infarct cortex and striatum where images were taken. (B) Representative images showing the co-expressed cells of Ki67+/NG2+ and Ki67+/CNPase+. (C) Quantitative data of the number of Ki67+/NG2+ and Ki67+/CNPase+ co-expressed cells in the peri-infarct cortex and striatum. (D,E) Representative images of Ki67/NG2 and Ki67/CNPase double immunofluorescent staining in the peri-infarct cortex and striatum. (F,G) Quantitative analysis of the protein and mRNA level of NG2 and CNPase in the peri-infarct brain tissues, respectively (one-way ANOVA followed by Bonferroni's post hoc test, n = 4 per group). ##P < 0.01 vs. control group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle group. ∧P < 0.05, ∧∧P < 0.01, ∧∧∧P < 0.001 vs. XSEC + EE group.

false