Mouse Recombinant Monoclonal NG2 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-NG2 antibody [7.1] - BSA and Azide free (AB279351), expandable thumbnail](https://content.abcam.com/products/images/ng2-antibody-71-bsa-and-azide-free-ab279351--flow-cytometry-img13550.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-NG2 antibody [7.1] - BSA and Azide free (AB279351)")
![Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [7.1] - BSA and Azide free (AB279351), expandable thumbnail](https://content.abcam.com/products/images/ng2-antibody-71-bsa-and-azide-free-ab279351--immunocytochemistry-immunofluorescence-img77633.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [7.1] - BSA and Azide free (AB279351)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Alternative names
MCSP, CSPG4, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan
Recommended products
Mouse Recombinant Monoclonal NG2 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 7.1
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab279351 is the carrier-free version of Anti-NG2 antibody [7.1] ab279348.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351), expandable thumbnail](https://content.abcam.com/products/images/ng2-antibody-71-bsa-and-azide-free-ab279351--flow-cytometry-img13550.jpg "Flow Cytometry - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)")
Flow Cytometry - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)
This data was developed using Anti-NG2 antibody [7.1] ab279348 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell, Left) / SK-MEL-28 (Human malignant melanoma, Right) cells labelling NG2 with Anti-NG2 antibody [7.1] ab279348 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: SK-BR-3.
Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351), expandable thumbnail](https://content.abcam.com/products/images/ng2-antibody-71-bsa-and-azide-free-ab279351--immunocytochemistry-immunofluorescence-img77633.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)")
Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [7.1] - BSA and Azide free (ab279351)
This data was developed using Anti-NG2 antibody [7.1] ab279348 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 cells labelling NG2 with Anti-NG2 antibody [7.1] ab279348 at 1/100 (11.02 μg/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in SK-MEL-28 cells.
Negative control: SK-BR-3 (PMID: 20852124).
Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: Anti-CSF-1-R antibody [YEYE311] ab271294 at a 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/1000 dilution.
Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at a 1/200 dilution followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157 at a 1/1000 dilution.
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