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Anti-NG2 antibody [EPR23976-145] (ab275024) is a rabbit monoclonal antibody that is used to detect NG2 in Western Blot, Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency


Images

Flow Cytometry - Anti-NG2 antibody [EPR23976-145] (AB275024), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (AB275024), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (AB275024), expandable thumbnail
  • Immunoprecipitation - Anti-NG2 antibody [EPR23976-145] (AB275024), expandable thumbnail
  • Western blot - Anti-NG2 antibody [EPR23976-145] (AB275024), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Tested
Expected
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Tested
Expected
Expected
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

Alternative names

Recommended products

Anti-NG2 antibody [EPR23976-145] (ab275024) is a rabbit monoclonal antibody that is used to detect NG2 in Western Blot, Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23976-145
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-NG2 antibody [EPR23976-145] (ab275024) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of NG2?


Anti-NG2 [EPR23976-145] (ab275024) specifically detects a band for NG2 (UniProt: Q8VHY0) at a molecular weight of 251kDa.

Recommended positive controls


WB: Human pancreas tissue lysate; Mouse brain and pancreas tissue lysates; rat brain tissue lysate; A375 and SK-MEL-28 whole cell lysates.ICC/IF: Mouse primary neural/glia cells; Rat primary neural/glia cells; LADMAC cells.Flow cyt: LADMAC cells; Mouse primary neural glia cells, A-375 cells.IP: Mouse brain tissue lysate.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR23976-145] also available for your convenience:
ab275024, Carrier free - Anti-NG2 antibody [EPR23976-145] - BSA and Azide free ab275038, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-NG2 antibody [EPR23976-145] ab283639, PE - PE Anti-NG2 antibody [EPR23976-145] ab314252



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Flow Cytometry - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Flow Cytometry - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Flow cytometric analysis of Mouse primary neural glia cell cells labelling NG2 with ab275024 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).

    Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024)

    ab275024 staining NG2 in A375 cells, with negative expression in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab275024 at 5 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cell cells labelling NG2 with ab275024 at 1/100 (4.67 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in mouse primary glia cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. is observed. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    ve control 1: ab275024 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Immunoprecipitation - Anti-NG2 antibody [EPR23976-145] (ab275024)

    NG2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab275024 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275024 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: ab275024 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab275024 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    Sample loaded onto lane 1 was non-boiled as boiling may cause protein aggregates.

    All lanes: Immunoprecipitation - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Predicted band size: 251 kDa

    Observed band size: 280 kDa, 330 kDa

  • Western blot - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Western blot - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 59 seconds; Lane 4: 81 seconds.

    The band of 330KDa represents the intact NG2 proteoglycan modified by chondroitin sulfate, the band of 280KDa represents NG2 core protein.
    The molecular weight observed is consistent with what has been described in the literature (PMID: 20455858, 16625365, 23481707).

    Negative control: Mouse liver (PMID: 23481707).

    Samples are non-boiled as boiling may cause protein aggregates.

    All lanes: Western blot - Anti-NG2 antibody [EPR23976-145] (ab275024) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse pancreas tissue lysate at 40 µg

    Lane 4: Rat brain tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 251 kDa

    Observed band size: 280 kDa, 330 kDa

  • Flow Cytometry - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Flow Cytometry - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Flow cytometric analysis of LADMAC (Mouse bone marrow monocyte macrophage) cells labelling NG2 with ab275024 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LADMAC cells labelling NG2 with ab275024 at 1/50 (9.34 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in LADMAC cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Western blot - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds; Lane 2: 59 seconds; Lane 3: 125 seconds.

    The band of 330KDa represents the intact NG2 proteoglycan modified by chondroitin sulfate, the band of 280KDa represents NG2 core protein.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 20455858, 16625365, 23481707).

    Samples are non-boiled as boiling may cause protein aggregates.

    All lanes: Western blot - Anti-NG2 antibody [EPR23976-145] (ab275024) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SK-MEL-28 (human malignant melanoma) whole cell lysate at 20 µg

    Lane 3: Human pancreas tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 251 kDa

    Observed band size: 280 kDa, 330 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cell cells labelling NG2 with ab275024 at 1/100 (4.67 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing positive staining in rat primary glia cells.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain MAP2 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab275024 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

    -ve control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-NG2 antibody [EPR23976-145] (ab275024), expandable thumbnail

    Flow Cytometry - Anti-NG2 antibody [EPR23976-145] (ab275024)

    Flow cytometry overlay histogram showing left A-375 positive cells and right negative MCF7 stained with ab275024 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactionfollowed by the antibody (ab275024) (1x 106 in 100μl at 5.0 μg/ml (1/402)) for 30min on ice.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

    Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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