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AB139406

Anti-NG2 antibody [EPR9195]

5

(6 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal NG2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

MCSP, CSPG4, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] (AB139406)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] (AB139406)

Immunohistochemical analysis of paraffin-embedded Human blood vessel tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] (AB139406)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] (AB139406)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling NG2 with purified ab139406 at 1 : 400 dilution (1.33 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] (AB139406)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NG2 antibody [EPR9195] (AB139406)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labelling NG2 with unpurified ab139406 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-NG2 antibody [EPR9195] (AB139406)
  • WB

Unknown

Western blot - Anti-NG2 antibody [EPR9195] (AB139406)

All lanes:

Western blot - Anti-NG2 antibody [EPR9195] (ab139406) at 1/1000 dilution

All lanes:

A375 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 251 kDa

false

Western blot - Anti-NG2 antibody [EPR9195] (AB139406)
  • WB

Lab

Western blot - Anti-NG2 antibody [EPR9195] (AB139406)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NG2 antibody [EPR9195] (ab139406) at 1/2000 dilution

All lanes:

A375 (Human malignant melanoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 101 kDa,243 kDa,251 kDa,262 kDa

Observed band size: 245 kDa,270 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9195

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100 - 1/500", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p><strong>For unpurified format use at 1/1000 - 1/10000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
See full target information CSPG4
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 14:464-479 PubMed36860926

2023

Different Expression and Clinical Implications of Cancer-Associated Fibroblast (CAF) Markers in Brain Metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Md Rashedunnabi Akanda,Eun-Jung Ahn,Yeong Jin Kim,S M Abdus Salam,Myung-Giun Noh,Sung Sun Kim,Tae-Young Jung,In-Young Kim,Chang-Hyun Kim,Kyung-Hwa Lee,Kyung-Sub Moon

Thyroid : official journal of the American Thyroid Association 31:1481-1493 PubMed34078123

2021

CSPG4 Is a Potential Therapeutic Target in Anaplastic Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin E Egan,Dessislava Stefanova,Adnan Ahmed,Vijay J Raja,Jessica W Thiesmeyer,Kevin J Chen,Jacques A Greenberg,Taotao Zhang,Bing He,Brendan M Finnerty,Rasa Zarnegar,Moonsoo M Jin,Theresa Scognamiglio,Noah Dephoure,Thomas Fahey,Irene M Min

Breast cancer research : BCR 22:124 PubMed33176887

2020

Immuno-priming durvalumab with bevacizumab in HER2-negative advanced breast cancer: a pilot clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Quintela-Fandino,Esther Holgado,Luis Manso,Serafin Morales,Begoña Bermejo,Ramon Colomer,Juan V Apala,Raquel Blanco,Manuel Muñoz,Eduardo Caleiras,Vega Iranzo,Mario Martinez,Orlando Dominguez,Javier Hornedo,Lucia Gonzalez-Cortijo,Javier Cortes,Ariadna Gasol Cudos,Diego Malon,Antonio Lopez-Alonso,María C Moreno-Ortíz,Silvana Mouron,Santos Mañes

Clinical cancer research : an official journal of the American Association for Cancer Research 26:35-45 PubMed31597662

2019

Randomized Phase 0/I Trial of the Mitochondrial Inhibitor ME-344 or Placebo Added to Bevacizumab in Early HER2-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Quintela-Fandino,Serafín Morales,Alfonso Cortés-Salgado,Luis Manso,Juan V Apala,Manuel Muñoz,Ariadna Gasol Cudos,Joel Salla Fortuny,María Gion,Antonio Lopez-Alonso,Javier Cortés,Juan Guerra,Diego Malón,Eduardo Caleiras,Francisca Mulero,Silvana Mouron

Stem cell research & therapy 10:179 PubMed31215503

2019

Transplantation of human endometrial perivascular cells with elevated CYR61 expression induces angiogenesis and promotes repair of a full-thickness uterine injury in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongxun Li,Guijun Yan,Qiang Diao,Fei Yu,Xin'an Li,Xiaoqiang Sheng,Yong Liu,Yimin Dai,Huaijun Zhou,Xin Zhen,Yali Hu,Bruno Péault,Lijun Ding,Haixiang Sun,Hairong Li

Frontiers in microbiology 9:1483 PubMed30022975

2018

Expression and (Lacking) Internalization of the Cell Surface Receptors of Toxin B.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis Schöttelndreier,Katrin Seeger,Guntram A Grassl,Markus R Winny,Robert Lindner,Harald Genth

Stem cells international 2017:3175748 PubMed28874910

2017

Different Angiogenic Potentials of Mesenchymal Stem Cells Derived from Umbilical Artery, Umbilical Vein, and Wharton's Jelly.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Xu,Jianjun Zhou,Jingyu Liu,Yong Liu,Lei Wang,Ruiwei Jiang,Zhenyu Diao,Guijun Yan,Bruno Pèault,Haixiang Sun,Lijun Ding

Nature 538:350-355 PubMed27680706

2016

Frizzled proteins are colonic epithelial receptors for C. difficile toxin B.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Tao,Jie Zhang,Paul Meraner,Alessio Tovaglieri,Xiaoqian Wu,Ralf Gerhard,Xinjun Zhang,William B Stallcup,Ji Miao,Xi He,Julian G Hurdle,David T Breault,Abraham L Brass,Min Dong
View all publications
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Product promise

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