AB20156

Anti-NG2 antibody [LHM 2]

Name: Anti-NG2 antibody [LHM 2] (ab20156) | Abcam
Brand: Abcam Limited
SKU: AB20156
Price: 525 USD
Availability: InStock
Rating: 2 (2 reviews)

(2 Reviews)

(13 Publications)

Mouse Monoclonal NG2 antibody. Suitable for IP, Flow Cyt, WB, IHC-FoFr, IHC-Fr and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Cell preparation containing CSPG4 protein.

View Alternative Names

MCSP, CSPG4, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LHM 2

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-Fr, Flow Cyt, IP, IHC-FoFr

applications

Immunogen

Cell preparation containing CSPG4 protein. The exact immunogen used to generate this antibody is proprietary information.

Q6UVK1

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Reactivity data

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Product details

The LHM2 antibody is a very good marker for melanoma (>90% stained). LHM2 also stains basal cell carcinomas and subpopulation of basal keratinocytes. LHM2 can be used as a diagnostic panel for immunopathology of malignant melanoma and potential candidates for immunoimaging and immunotherapy of disseminated melanoma. The originators of this antibody report that the MCSP is a cell surface marker specific for epidermal stem cells (J. Invest. Dermatol. in press). Potential applications of the LHM2 antibody include use for separation/isolation of epidermal stem cells, detection of stem cells in skin biopsies or epithelial cell cultures and optimisation of culture conditions to retain epidermal stem cell population in in vitro cultures.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

See full target information CSPG4

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cells 8: PubMed31466397

2019

Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Sara R Bang-Christensen,Rasmus S Pedersen,Marina A Pereira,Thomas M Clausen,Caroline Løppke,Nicolai T Sand,Theresa D Ahrens,Amalie M Jørgensen,Yi Chieh Lim,Louise Goksøyr,Swati Choudhary,Tobias Gustavsson,Robert Dagil,Mads Daugaard,Adam F Sander,Mathias H Torp,Max Søgaard,Thor G Theander,Olga Østrup,Ulrik Lassen,Petra Hamerlik,Ali Salanti,Mette Ø Agerbæk

Stem cells translational medicine 5:1268-76 PubMed27388242

2016

Perivascular Progenitor Cells Derived From Human Embryonic Stem Cells Exhibit Functional Characteristics of Pericytes and Improve the Retinal Vasculature in a Rodent Model of Diabetic Retinopathy.

Applications

Unspecified application

Species

Human

Jung Mo Kim,Ki-Sung Hong,Won Kyung Song,Daekyeong Bae,In-Kyu Hwang,Jong Soo Kim,Hyung-Min Chung

Journal of applied oral science : revista FOB 23:79-86 PubMed25760270

2015

Differential expression of stem cell-like proteins in normal, hyperplastic and dysplastic oral epithelium.

Applications

IHC

Species

Unspecified reactive species

Sarah Mohammed Mohammed Barakat,Chong Huat Siar

BioMed research international 2014:395781 PubMed24563864

2014

Direct cell-cell contact between mesenchymal stem cells and endothelial progenitor cells induces a pericyte-like phenotype in vitro.

Applications

ICC/IF

Species

Unspecified reactive species

Markus Loibl,Andreas Binder,Marietta Herrmann,Fabian Duttenhoefer,R Geoff Richards,Michael Nerlich,Mauro Alini,Sophie Verrier

Clinical cancer research : an official journal of 18:5479-88 PubMed22855580

2012

The relationship between homologous recombination repair and the sensitivity of human epidermis to the size of daily doses over a 5-week course of breast radiotherapy.

Applications

IHC-P

Species

Human

Navita Somaiah,John Yarnold,Frances Daley,Ann Pearson,Lone Gothard,Kai Rothkamm,Thomas Helleday

Stem cells and development 21:1794-802 PubMed21970342

2011

Transplantation of undifferentiated and induced human exfoliated deciduous teeth-derived stem cells promote functional recovery of rat spinal cord contusion injury model.

Applications

IHC-FoFr

Species

Rat

Zahra Taghipour,Khadijeh Karbalaie,Abbas Kiani,Ali Niapour,Hamid Bahramian,Mohammad Hossein Nasr-Esfahani,Hossein Baharvand

FASEB journal : official publication of the Federa 25:2956-66 PubMed21602448

2011

Activation of nuclear factor-κB following muscle eccentric contractions in humans is localized primarily to skeletal muscle-residing pericytes.

Applications

IHC-P

Species

Human

Robert D Hyldahl,Ling Xin,Monica J Hubal,Stephanie Moeckel-Cole,Stuart Chipkin,Priscilla M Clarkson

Arteriosclerosis, thrombosis, and vascular biology 30:1220-7 PubMed20299690

2010

Vascular endothelial growth factor-secreting mast cells and myofibroblasts: a novel self-perpetuating angiogenic pathway in aortic valve stenosis.

Applications

IHC-Fr

Species

Human

Suvi Syväranta,Satu Helske,Mika Laine,Jani Lappalainen,Markku Kupari,Mikko I Mäyränpää,Ken A Lindstedt,Petri T Kovanen

Arthritis and rheumatism 60:2741-6 PubMed19714645

2009

Type II collagen levels correlate with mineralization by articular cartilage vesicles.

Applications

WB, WB

Species

Human, Pig

Brian Jubeck,Emily Muth,Claudia M Gohr,Ann K Rosenthal

The Journal of cell biology 177:87-101 PubMed17420292

2007

Evidence for a stem cell hierarchy in the adult human breast.

Applications

Unspecified application

Species

Unspecified reactive species

René Villadsen,Agla J Fridriksdottir,Lone Rønnov-Jessen,Thorarinn Gudjonsson,Fritz Rank,Mark A LaBarge,Mina J Bissell,Ole W Petersen

View all publications

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