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AB168351

Anti-NgBR antibody [EPR8668]

5

(1 Review)

|

(13 Publications)

Rabbit Recombinant Monoclonal NGBR antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications.

View Alternative Names

C6orf68, NGBR, NUS1, Dehydrodolichyl diphosphate synthase complex subunit NUS1, Cis-prenyltransferase subunit NgBR, Nogo-B receptor, Nuclear undecaprenyl pyrophosphate synthase 1 homolog, NgBR

1 Images
Western blot - Anti-NgBR antibody [EPR8668] (AB168351)
  • WB

Unknown

Western blot - Anti-NgBR antibody [EPR8668] (AB168351)

All lanes:

Western blot - Anti-NgBR antibody [EPR8668] (ab168351) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8668

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

This antibody was developed as part of a collaboration between Epitomics, the National Institutes of Health and the lab of Qing (Robert) Miao from the Medical College of Wisconsin.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NgBR also known as the Nogo-B receptor is a transmembrane protein with an approximate mass of 38 kDa. It plays a role in cellular mechanisms especially within endothelial cells where it is expressed abundantly. NgBR involves itself in modulating the activity and localization of certain enzymes particularly in processes requiring lipid modification. Expression of NgBR can also be found in other tissues suggesting a broader range of functions.
Biological function summary

NgBR engages in various cellular operations influencing development survival and migration of cells. It acts as a cofactor for enzymes participating in dolichol biosynthesis and cholesterol synthesis. NgBR often forms a complex with other proteins to execute these roles. By interacting with Nogo-B NgBR impacts key angiogenesis and vascular homeostasis decisions playing part in growth and maintenance of blood vessels.

Pathways

NgBR aligns itself with the PI3K-Akt signaling cascade and the Ras pathway both important in cellular proliferation and survival. In these pathways NgBR associates closely with PI3K and interacts with Ras proteins enabling effective signal transduction. NgBR's contribution helps maintain cellular responses to growth signals and ensures proper functioning of cellular mechanisms.

Altered expression or function of NgBR associates with various pathologies including cancer and cardiovascular diseases. NgBR's connection to cancer lies in its involvement with endothelial survival and proliferation with disruptions potentially leading to tumor growth and metastasis. In cardiovascular disease NgBR plays a role in endothelial dysfunction linking with proteins such as Nogo-B which influences blood vessel repair and formation hinting at implications in atherosclerosis and related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery (PubMed : 21572394, PubMed : 25066056, PubMed : 28842490, PubMed : 32817466, PubMed : 33077723). Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER) (PubMed : 21572394, PubMed : 25066056, PubMed : 28842490, PubMed : 32817466, PubMed : 33077723). Synthesizes long-chain polyprenols, mostly of C95 and C100 chain length (PubMed : 32817466). Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol (PubMed : 21572394). Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator (PubMed : 16835300).
See full target information NUS1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmacologica Sinica 44:2216-2229 PubMed37402997

2023

6-Methyl flavone inhibits Nogo-B expression and improves high fructose diet-induced liver injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Gong,Zhen Zhang,Sha-Sha Chen,Xin-Ran Zhu,Meng-Yao Wang,Xin-Yue Yang,Chen Ding,Ji-Hong Han,Qing-Shan Li,Ya-Jun Duan

The Journal of biological chemistry 298:101561 PubMed34998825

2022

Inhibition of high-fat diet-induced obesity via reduction of ER-resident protein Nogo occurs through multiple mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolin Wang,Yanfang Yang,Dan Zhao,Shuang Zhang,Yi Chen,Yuanli Chen,Ke Feng,Xiaoju Li,Jihong Han,Yasuko Iwakiri,Yajun Duan,Xiaoxiao Yang

American journal of human genetics 108:722-738 PubMed33798445

2021

Progressive myoclonus epilepsies-Residual unsolved cases have marked genetic heterogeneity including dolichol-dependent protein glycosylation pathway genes.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Courage,Karen L Oliver,Eon Joo Park,Jillian M Cameron,Kariona A Grabińska,Mikko Muona,Laura Canafoglia,Antonio Gambardella,Edith Said,Zaid Afawi,Betul Baykan,Christian Brandt,Carlo di Bonaventura,Hui Bein Chew,Chiara Criscuolo,Leanne M Dibbens,Barbara Castellotti,Patrizia Riguzzi,Angelo Labate,Alessandro Filla,Anna T Giallonardo,Geza Berecki,Christopher B Jackson,Tarja Joensuu,John A Damiano,Sara Kivity,Amos Korczyn,Aarno Palotie,Pasquale Striano,Davide Uccellini,Loretta Giuliano,Eva Andermann,Ingrid E Scheffer,Roberto Michelucci,Melanie Bahlo,Silvana Franceschetti,William C Sessa,Samuel F Berkovic,Anna-Elina Lehesjoki

Genetics in medicine : official journal of the American College of Medical Genetics 23:1305-1314 PubMed33731878

2021

Lysosomal cholesterol accumulation contributes to the movement phenotypes associated with NUS1 haploinsufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Seok-Ho Yu,Tong Wang,Kali Wiggins,Raymond J Louie,Emilio F Merino,Cindy Skinner,Maria B Cassera,Kirsten Meagher,Paul Goldberg,Neggy Rismanchi,Dillon Chen,Michael J Lyons,Heather Flanagan-Steet,Richard Steet

Frontiers in pharmacology 12:615889 PubMed33716742

2021

Artesunate Regulates Neurite Outgrowth Inhibitor Protein B Receptor to Overcome Resistance to Sorafenib in Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wubin He,Xiaoxu Huang,Bradford K Berges,Yue Wang,Ni An,Rongjian Su,Yanyan Lu

PloS one 14:e0220715 PubMed31442237

2019

Silencing Nogo-B receptor inhibits penile corpus cavernosum vascular smooth muscle cell apoptosis of rats with diabetic erectile dysfunction by down-regulating ICAM-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Wei Huo,Yan Wen,Hai Li

International journal of molecular sciences 20: PubMed31083380

2019

Nogo-B Receptor Directs Mitochondria-Associated Membranes to Regulate Vascular Smooth Muscle Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Dong Yang,Man-Man Li,Gang Xu,Lan Feng,Er-Long Zhang,Jian Chen,De-Wei Chen,Yu-Qi Gao

Breast cancer research : BCR 20:112 PubMed30208932

2018

Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pin Gao,Xiang Wang,Ying Jin,Wenquan Hu,Yajun Duan,Aiping Shi,Ye Du,Dong Song,Ming Yang,Sijie Li,Bing Han,Gang Zhao,Hongquan Zhang,Zhimin Fan,Qing Robert Miao

Cancer letters 419:233-244 PubMed29373839

2018

Nogo-B receptor increases the resistance of estrogen receptor positive breast cancer to paclitaxel.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Jin,Wenquan Hu,Tong Liu,Ujala Rana,Irene Aguilera-Barrantes,Amanda Kong,Suresh N Kumar,Bei Wang,Pin Gao,Xiang Wang,Yajun Duan,Aiping Shi,Dong Song,Ming Yang,Sijie Li,Bing Han,Gang Zhao,Zhimin Fan,Qing Robert Miao

The Journal of biological chemistry 292:17351-17361 PubMed28842490

2017

A conserved C-terminal RG motif in the NgBR subunit of -prenyltransferase is critical for prenyltransferase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Kariona A Grabińska,Ban H Edani,Eon Joo Park,Jan R Kraehling,William C Sessa
View all publications
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