Anti-NGF antibody - BSA and Azide free

• WB

Unknown

Western blot - Anti-NGF antibody - BSA and Azide free (AB6199)

All lanes:

Western blot - Anti-NGF antibody - BSA and Azide free (ab6199) at 1 µg/mL

All lanes:

Active human NGF full length protein (<a href='/en-us/products/unavailable/active-human-ngf-full-length-protein-ab69759'>ab69759</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 26 kDa

true

Exposure time: 30s

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody - BSA and Azide free (AB6199)

ICC/IF image of ab6199 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6199, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

Supplier Data

Western blot - Anti-NGF antibody - BSA and Azide free (AB6199)

ab6199 detects a strong band at 13 kDa consistent with the expected molecular weight of mature NGF monomer.

All lanes:

Western blot - Anti-NGF antibody - BSA and Azide free (ab6199) at 5 µg/mL

Lane 1:

rhNGF protein at 100 µg

Lane 2:

Mouse salivary gland tissue lysate at 50 µg

Predicted band size: 26 kDa

false