AB174323

Anti-NgR3 antibody [EPR11334]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal NgR3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NGRH2, NGRL2, RTN4RL1, Reticulon-4 receptor-like 1, Nogo receptor-like 2, Nogo-66 receptor homolog 2, Nogo-66 receptor-related protein 3, NgR3

2 Images

Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (AB174323)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (AB174323)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling NgR3 with ab174323 at 1/100 dilution (red) compared with a rabbit IgG negative control (green).

Supplier Data

Western blot - Anti-NgR3 antibody [EPR11334] (AB174323)

All lanes:

Western blot - Anti-NgR3 antibody [EPR11334] (ab174323) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11334

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NgR3 also known as Nogo-66 Receptor Homolog 3 or Reticulon 4 Receptor-Like 2 is part of the Nogo receptor family. It is a glycolipid-anchored receptor weighing approximately 55 kDa. This protein is mainly found in the central nervous system and is widely expressed in the brain though it also appears in other tissues to a lesser extent. NgR3 contributes to axon growth inhibition and neuronal regeneration influencing nerve repair processes.

Biological function summary

NgR3 influences axonal guidance and inhibition by interacting with other proteins. It forms part of a receptor complex typically incorporating p75 neurotrophin receptor and Lingo-1 which are important for signaling processes. Through these interactions NgR3 helps moderate the effects of myelin-associated inhibitors on axonal outgrowth supporting the regulation of neuronal plasticity and myelin repair mechanisms.

Pathways

This receptor plays a significant role in the regulation of axon regeneration. It intersects with the Nogo-A signaling pathway where it works with closely related proteins such as NgR1 and NgR2. These interactions inhibit axonal growth and impact neuronal development and recovery post-injury. Through this pathway NgR3 aids in balancing axonal regeneration and pathfinding during neural development and repair.

NgR3 is associated with conditions that involve axonal damage and neurodegeneration including multiple sclerosis and spinal cord injuries. It impacts these conditions by modulating axonal growth inhibitory mechanisms. In multiple sclerosis NgR3 works with proteins like Nogo-A to influence remyelination processes potentially affecting the disease progression. Understanding the role of NgR3 in these contexts could aid in developing therapeutic strategies aimed at enhancing neural repair and recovery.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cell surface receptor. Plays a functionally redundant role in postnatal brain development and in regulating axon regeneration in the adult central nervous system. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG. Plays a role in inhibiting neurite outgrowth and axon regeneration via its binding to neuronal chondroitin sulfate proteoglycans. Binds heparin (By similarity). Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development (PubMed : 22325200). Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton (PubMed : 22325200).

See full target information RTN4RL1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:880382 PubMed35592113

2022

Stress Elicits Contrasting Effects on Rac1-Cofilin Signaling in the Hippocampus and Amygdala.

Applications

Unspecified application

Species

Unspecified reactive species

Mihika Bose,Mohammad Sarfaraz Nawaz,Rakhi Pal,Sumantra Chattarji

Saudi journal of biological sciences 28:4132-4136 PubMed34354392

2021

Effect of ganglioside combined with Chip Jiaji electro-acupuncture on Nogo-NgR signal pathway in SCI rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfeng Hu,Hui Wang,Wei Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NgR3 antibody [EPR11334]

Flow Cytometry (Intracellular) - Anti-NgR3 antibody [EPR11334] (AB174323)

Western blot - Anti-NgR3 antibody [EPR11334] (AB174323)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise