AB151443

Anti-NHERF-2/SIP-1 antibody [32B6]

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(2 Publications)

Mouse Monoclonal NHERF-2/SIP-1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NHERF2.

View Alternative Names

SLC9A3R2, NHERF2, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHERF-2, NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1, Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2, Tyrosine kinase activator protein 1, SIP-1, TKA-1

2 Images

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Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (AB151443)

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Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443) at 1/100 dilution

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HaCat Human keratinocyte cells

Predicted band size: 37 kDa

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Lab

Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (AB151443)

Lanes 1-4 : Merged signal (red and green). Green - ab151443 observed at 45 kDa. Red - loading control ab52901 observed at kDa.

ab151443 Anti-NHERF-2/SIP-1 antibody [32B6] was shown to specifically react with NHERF-2/SIP-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266778 (knockout cell lysate ab258685) was used. Wild-type and NHERF-2/SIP-1 knockout samples were subjected to SDS-PAGE. ab151443 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (ab151443) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

SLC9A3R2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human SLC9A3R2 (NHERF-2/SIP-1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-slc9a3r2-nherf-2-sip-1-knockout-hek-293t-cell-line-ab266778'>ab266778</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

T-47D cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 37 kDa

Observed band size: 45 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

32B6

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NHERF2.

Q15599

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3 (PubMed : 18829453). May also act as scaffold protein in the nucleus.

See full target information NHERF2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39940923

2025

NHERF2 as a Novel Biomarker for Distinguishing MAC Pulmonary Disease from Tuberculosis Based on Proteome Analysis of Serum Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Maiko Naito,Yoshito Takeda,Ryuya Edahiro,Yuya Shirai,Takatoshi Enomoto,Mana Nakayama,Satoshi Nojima,Mari Nogami-Ito,Masahide Mori,Yukihiro Yano,Takanori Matsuki,Hanako Yoshimura,Reina Hara,Makoto Yamamoto,Kentaro Masuhiro,Yujiro Naito,Shohei Koyama,Kota Iwahori,Izumi Nagatomo,Takayuki Shiroyama,Kotaro Miyake,Haruhiko Hirata,Hiroaki Hase,Kazutake Tsujikawa,Koji Ueda,Atsushi Kumanogoh

Nature communications 6:10131 PubMed26647728

2015

Frequent alterations in cytoskeleton remodelling genes in primary and metastatic lung adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Wu,Xin Zhang,Fuqiang Li,Dakai Xiao,Yong Hou,Shida Zhu,Dongbing Liu,Xiaofei Ye,Mingzhi Ye,Jie Yang,Libin Shao,Hui Pan,Na Lu,Yuan Yu,Liping Liu,Jin Li,Liyan Huang,Hailing Tang,Qiuhua Deng,Yue Zheng,Lihua Peng,Geng Liu,Xia Gu,Ping He,Yingying Gu,Weixuan Lin,Huiming He,Guoyun Xie,Han Liang,Na An,Hui Wang,Manuel Teixeira,Joana Vieira,Wenhua Liang,Xin Zhao,Zhiyu Peng,Feng Mu,Xiuqing Zhang,Xun Xu,Huanming Yang,Karsten Kristiansen,Jian Wang,Nanshan Zhong,Jun Wang,Qiang Pan-Hammarström,Jianxing He

View all publications

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Product promise

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Anti-NHERF-2/SIP-1 antibody [32B6]

Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (AB151443)

Western blot - Anti-NHERF-2/SIP-1 antibody [32B6] (AB151443)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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