Rabbit Recombinant Monoclonal NHP2 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-NHP2 antibody [EPR12404] (AB180498), expandable thumbnail](https://content.abcam.com/products/images/nhp2-antibody-epr12404-ab180498--western-blot-img108450.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NHP2 antibody [EPR12404] (AB180498)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] (AB180498), expandable thumbnail](https://content.abcam.com/products/images/nhp2-antibody-epr12404-ab180498--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109224.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] (AB180498)")
![Flow Cytometry (Intracellular) - Anti-NHP2 antibody [EPR12404] (AB180498), expandable thumbnail](https://content.abcam.com/products/images/nhp2-antibody-epr12404-ab180498--flow-cytometry-intracellular-img50571.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NHP2 antibody [EPR12404] (AB180498)")
Publications
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- Molecular & cellular proteomics : MCP 20:1001152021Age-Associated Proteomic Signatures and Potential Clinically Actionable Targets of Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanqiu Gong,Yu Liu,Tian Wang,Zhigui Li,Li Gao,Haining Chen,Yang Shu,Yuan Li,Heng Xu,Zongguang Zhou,Lunzhi DaiPubMed 34129943
- EMBO reports 22:e506842021The deubiquitinase USP36 promotes snoRNP group SUMOylation and is essential for ribosome biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyunju Ryu,Xiao-Xin Sun,Yingxiao Chen,Yanping Li,Xiaoyan Wang,Roselyn S Dai,Hong-Ming Zhu,John Klimek,Larry David,Lev M Fedorov,Yoshiaki Azuma,Rosalie C Sears,Mu-Shui DaiPubMed 33852194
- Aging 12:19365-193742020Knockdown of NHP2 inhibits hepatitis B virus X protein-induced hepatocarcinogenesis via repressing TERT expression and disrupting the stability of telomerase complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuming Tang et. al.PubMed 33044946
- Human molecular genetics 29:907-9222020NHP2 deficiency impairs rRNA biogenesis and causes pulmonary fibrosis and Høyeraal-Hreidarsson syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManame Benyelles,Marie-Françoise O'Donohue,Laëtitia Kermasson,Elodie Lainey,Raphael Borie,Chantal Lagresle-Peyrou,Hilario Nunes,Clarisse Cazelles,Cécile Fourrage,Emmanuelle Ollivier,Ambroise Marcais,Anne-Sophie Gamez,Fanny Morice-Picard,Denis Caillaud,Nicolas Pottier,Christelle Ménard,Ibrahima Ba,Alicia Fernandes,Bruno Crestani,Jean-Pierre de Villartay,Pierre-Emmanuel Gleizes,Isabelle Callebaut,Caroline Kannengiesser,Patrick RevyPubMed 31985013
- Journal of enzyme inhibition and medicinal chemistry 33:1199-12112018Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Quan Wang,Meng Di Yang,Xing Chen,Yang Wang,Liu Zeng Chen,Xiu Cheng,Xin Hua LiuPubMed 30132373
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Alternative names
NOLA2, HSPC286, NHP2, H/ACA ribonucleoprotein complex subunit 2, Nucleolar protein family A member 2, snoRNP protein NHP2
Recommended products
Rabbit Recombinant Monoclonal NHP2 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12404
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-NHP2 antibody [EPR12404] (ab180498), expandable thumbnail](https://content.abcam.com/products/images/nhp2-antibody-epr12404-ab180498--western-blot-img108450.jpg "Western blot - Anti-NHP2 antibody [EPR12404] (ab180498)")
Western blot - Anti-NHP2 antibody [EPR12404] (ab180498)
All lanes: Western blot - Anti-NHP2 antibody [EPR12404] (ab180498) at 1/2000 dilution
Lane 1: K562 lysate at 20 µg
Lane 2: Jurkat lysate at 20 µg
Lane 3: Caco-2 cell lysate at 20 µg
Lane 4: SH-SY5Y cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] (ab180498), expandable thumbnail](https://content.abcam.com/products/images/nhp2-antibody-epr12404-ab180498--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109224.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] (ab180498)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2 antibody [EPR12404] (ab180498)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling NHP2 with ab180498 at 1/500 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG and counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-NHP2 antibody [EPR12404] (ab180498), expandable thumbnail](https://content.abcam.com/products/images/nhp2-antibody-epr12404-ab180498--flow-cytometry-intracellular-img50571.jpg "Flow Cytometry (Intracellular) - Anti-NHP2 antibody [EPR12404] (ab180498)")
Flow Cytometry (Intracellular) - Anti-NHP2 antibody [EPR12404] (ab180498)
Intracellular flow cytometric analysis of K562 cells (2% paraformaldehyde-fixed)labeling NHP2 with ab180498 at 1/20 (red) compared to a Rabbit monoclonal IgGnegative control antibody (green) followed byGoat anti rabbit IgG (FITC) at 1/75 dilution.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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