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AB95958

Anti-NHP2L1 antibody

4

(3 Reviews)

|

(5 Publications)

Rabbit Polyclonal NHP2L1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNU13 aa 1-100.

View Alternative Names

NHP2L1, SNU13, NHP2-like protein 1, High mobility group-like nuclear protein 2 homolog 1, OTK27, SNU13 homolog, U4/U6.U5 small nuclear ribonucleoprotein SNU13, U4/U6.U5 tri-snRNP 15.5 kDa protein, hSNU13

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody (AB95958)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody (AB95958)

ab95958, at a 1/100 dilution, staining NHP2L1 in paraffin embedded Cal27 xenograft by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody (AB95958)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody (AB95958)

ab95958, at a 1/200 dilution, staining NHP2L1 in paraformaldehyde fixed HeLa cell by Immunofluorescence analysis.

Western blot - Anti-NHP2L1 antibody (AB95958)
  • WB

Unknown

Western blot - Anti-NHP2L1 antibody (AB95958)

12% SDS PAGE

All lanes:

Western blot - Anti-NHP2L1 antibody (ab95958) at 1/1000 dilution

Lane 1:

Hela whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 14 kDa

false

Western blot - Anti-NHP2L1 antibody (AB95958)
  • WB

Supplier Data

Western blot - Anti-NHP2L1 antibody (AB95958)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-NHP2L1 antibody (ab95958) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

GL261 whole cell lysates at 30 µg

Lane 3:

C8D30 whole cell lysates at 30 µg

Lane 4:

NIH-3T3 whole cell lysates at 30 µg

Lane 5:

BCL-1 whole cell lysates at 30 µg

Lane 6:

Raw 264.7 whole cell lysates at 30 µg

Lane 7:

C2Cl2 whole cell lysates at 30 µg

Predicted band size: 14 kDa

false

Western blot - Anti-NHP2L1 antibody (AB95958)
  • WB

Supplier Data

Western blot - Anti-NHP2L1 antibody (AB95958)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-NHP2L1 antibody (ab95958) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SNU13 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P55769

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 28781166). Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly (PubMed : 10545122, PubMed : 17412961). The protein undergoes a conformational change upon RNA-binding (PubMed : 10545122, PubMed : 17412961, PubMed : 28781166).
See full target information SNU13
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational science 14:1490-1504 PubMed33742760

2021

Identification of small molecules that mitigate vincristine-induced neurotoxicity while sensitizing leukemia cells to vincristine.

Applications

Unspecified application

Species

Unspecified reactive species

Barthelemy Diouf,Claudia Wing,John C Panetta,Donnie Eddins,Wenwei Lin,Wenjian Yang,Yiping Fan,Deqing Pei,Cheng Cheng,Shannon M Delaney,Wei Zhang,Erik J Bonten,Kristine R Crews,Steven W Paugh,Lie Li,Burgess B Freeman,Robert J Autry,Jordan A Beard,Daniel C Ferguson,Laura J Janke,Kirsten K Ness,Taosheng Chen,Stanislav S Zakharenko,Sima Jeha,Ching-Hon Pui,Mary V Relling,M Eileen Dolan,William E Evans

Journal of experimental & clinical cancer research : CR 40:82 PubMed33648524

2021

Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention.

Applications

Unspecified application

Species

Unspecified reactive species

Esmee Koedoot,Eline van Steijn,Marjolein Vermeer,Román González-Prieto,Alfred C O Vertegaal,John W M Martens,Sylvia E Le Dévédec,Bob van de Water

PLoS pathogens 12:e1005772 PubMed27454487

2016

Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response.

Applications

WB

Species

Unspecified reactive species

Nicolas Tremblay,Martin Baril,Laurent Chatel-Chaix,Salwa Es-Saad,Alex Young Park,Robert K Koenekoop,Daniel Lamarre

Proceedings of the National Academy of Sciences of 113:E1625-34 PubMed26957605

2016

Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Falaleeva,Amadis Pages,Zaneta Matuszek,Sana Hidmi,Lily Agranat-Tamir,Konstantin Korotkov,Yuval Nevo,Eduardo Eyras,Ruth Sperling,Stefan Stamm

The EMBO journal 33:2623-42 PubMed25257310

2014

Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion.

Applications

WB

Species

Unspecified reactive species

Sriramkumar Sundaramoorthy,María Dolores Vázquez-Novelle,Sergey Lekomtsev,Michael Howell,Mark Petronczki
View all publications
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