JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181982

Anti-NHP2L1 antibody [EPR11671]

5

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal NHP2L1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

NHP2L1, SNU13, NHP2-like protein 1, High mobility group-like nuclear protein 2 homolog 1, OTK27, SNU13 homolog, U4/U6.U5 small nuclear ribonucleoprotein SNU13, U4/U6.U5 tri-snRNP 15.5 kDa protein, hSNU13

5 Images
Flow Cytometry (Intracellular) - Anti-NHP2L1 antibody [EPR11671] (AB181982)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NHP2L1 antibody [EPR11671] (AB181982)

Intracellular Flow Cytometry analysis ofMCF-7 cells labeling NHP2L1 using ab181982 at 1/50 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] (AB181982)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] (AB181982)

Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling NHP2L1 with ab181982 at 1/1000 dilution followed by secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin counterstain.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] (AB181982)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] (AB181982)

Immunofluorescence analysis of HeLa cells labeling NHP2L1 with ab181982 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor® 555) (1/200 dilution) and counter stained with Dapi.

Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] (AB181982)
  • IP

Supplier Data

Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] (AB181982)

Immunoprecipitation analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/40 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] (ab181982)

Predicted band size: 14 kDa

false

Western blot - Anti-NHP2L1 antibody [EPR11671] (AB181982)
  • WB

Supplier Data

Western blot - Anti-NHP2L1 antibody [EPR11671] (AB181982)

All lanes:

Western blot - Anti-NHP2L1 antibody [EPR11671] (ab181982) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

MCF-7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

false

  • Carrier free

    Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11671

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 28781166). Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly (PubMed : 10545122, PubMed : 17412961). The protein undergoes a conformational change upon RNA-binding (PubMed : 10545122, PubMed : 17412961, PubMed : 28781166).
See full target information SNU13
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5237 PubMed38898005

2024

Therapy-induced secretion of spliceosomal components mediates pro-survival crosstalk between ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria O Shender,Ksenia S Anufrieva,Polina V Shnaider,Georgij P Arapidi,Marat S Pavlyukov,Olga M Ivanova,Irina K Malyants,Grigory A Stepanov,Evgenii Zhuravlev,Rustam H Ziganshin,Ivan O Butenko,Olga N Bukato,Ksenia M Klimina,Vladimir A Veselovsky,Tatiana V Grigorieva,Sergey Y Malanin,Olga I Aleshikova,Andrey V Slonov,Nataliya A Babaeva,Lev A Ashrafyan,Elena Khomyakova,Evgeniy G Evtushenko,Maria M Lukina,Zixiang Wang,Artemiy S Silantiev,Anna A Nushtaeva,Daria D Kharlampieva,Vassili N Lazarev,Arseniy I Lashkin,Lorine K Arzumanyan,Irina Yu Petrushanko,Alexander A Makarov,Olga S Lebedeva,Alexandra N Bogomazova,Maria A Lagarkova,Vadim M Govorun

Nature communications 12:4980 PubMed34404792

2021

Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Samuel A Myers,Dominique K Carey,Steven A Carr,Alice Y Ting

International journal of biological sciences 16:1388-1402 PubMed32210727

2020

The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xu,Minghua Huang,Jia Guo,Huiting Zhang,Depeng Wang,Tiantian Liu,Haiting Liu,Shiming Chen,Peng Gao,Kun Mu

Proceedings of the National Academy of Sciences of 114:E6749-E6758 PubMed28760965

2017

Quantitative time-resolved chemoproteomics reveals that stable -GlcNAc regulates box C/D snoRNP biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Pinou Lv,Xinqi Fan,Baiyi Quan,Yuntao Zhu,Ke Qin,Ying Chen,Chu Wang,Xing Chen
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB95958

Anti-NHP2L1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody (AB95958)

  • 4
  • 3

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB79237

Anti-PRPF8/Prp8 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody (AB79237)

  • 5
  • 5
Primary Antibodies

AB198291

Anti-SRSF3 antibody [EPR16976]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SRSF3 antibody [EPR16976] (AB198291)

  • 5
  • 6
Primary Antibodies

AB172614

Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (AB172614)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com