Rabbit Recombinant Monoclonal NHP2L1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Western blot - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--western-blot-img116245.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89627.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549)")
![Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--immunoprecipitation-img19684.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549)")
![Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--immunocytochemistry-immunofluorescence-img93617.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549)")
![Flow Cytometry (Intracellular) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--flow-cytometry-intracellular-img63422.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (AB250549)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797). Involved in pre-mRNA splicing as component of the spliceosome (PubMed:28781166). Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly (PubMed:10545122, PubMed:17412961). The protein undergoes a conformational change upon RNA-binding (PubMed:10545122, PubMed:17412961, PubMed:28781166).
Alternative names
NHP2L1, SNU13, NHP2-like protein 1, High mobility group-like nuclear protein 2 homolog 1, OTK27, SNU13 homolog, U4/U6.U5 small nuclear ribonucleoprotein SNU13, U4/U6.U5 tri-snRNP 15.5 kDa protein, hSNU13
Recommended products
Rabbit Recombinant Monoclonal NHP2L1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11671
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250549 is the carrier-free version of Anti-NHP2L1 antibody [EPR11671] ab181982.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--western-blot-img116245.jpg "Western blot - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)")
Western blot - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)
This data was developed using Anti-NHP2L1 antibody [EPR11671] ab181982, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NHP2L1 antibody [EPR11671] (Anti-NHP2L1 antibody [EPR11671] ab181982) at 1/20000 dilution
Lane 1: HepG2 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: MCF-7 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 14 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89627.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)
This data was developed using Anti-NHP2L1 antibody [EPR11671] ab181982, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling NHP2L1 with Anti-NHP2L1 antibody [EPR11671] ab181982 at 1/1000 dilution followed by secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin counterstain. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--immunoprecipitation-img19684.jpg "Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)")
Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)
This data was developed using Anti-NHP2L1 antibody [EPR11671] ab181982, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of MCF-7 cells labeling NHP2L1 using Anti-NHP2L1 antibody [EPR11671] ab181982 at 1/40 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
All lanes: Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] (Anti-NHP2L1 antibody [EPR11671] ab181982)
Predicted band size: 14 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--immunocytochemistry-immunofluorescence-img93617.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)")
Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)
This data was developed using Anti-NHP2L1 antibody [EPR11671] ab181982, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling NHP2L1 with Anti-NHP2L1 antibody [EPR11671] ab181982 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor® 555) (1/200 dilution) and counter stained with Dapi.
![Flow Cytometry (Intracellular) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549), expandable thumbnail](https://content.abcam.com/products/images/nhp2l1-antibody-epr11671-bsa-and-azide-free-ab250549--flow-cytometry-intracellular-img63422.jpg "Flow Cytometry (Intracellular) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)")
Flow Cytometry (Intracellular) - Anti-NHP2L1 antibody [EPR11671] - BSA and Azide free (ab250549)
This data was developed using Anti-NHP2L1 antibody [EPR11671] ab181982, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of MCF-7 cells labeling NHP2L1 using Anti-NHP2L1 antibody [EPR11671] ab181982 at 1/50 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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