AB248361

Anti-Nicalin antibody [EPR8528] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Nicalin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

BOS complex subunit NCLN, Nicalin, Nicastrin-like protein, NCLN

2 Images

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Western blot - Anti-Nicalin antibody [EPR8528] - BSA and Azide free (AB248361)

This data was developed using ab131159, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Nicalin antibody [EPR8528] (<a href='/en-us/products/primary-antibodies/nicalin-antibody-epr8528-ab131159'>ab131159</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

BxPC-3 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

Raw 264.7 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 63 kDa

Observed band size: 60 kDa

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OI-RD Scanning - Anti-Nicalin antibody [EPR8528] - BSA and Azide free (AB248361)

OI-RD Scanning

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OI-RD Scanning - Anti-Nicalin antibody [EPR8528] - BSA and Azide free (AB248361)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-Nicalin antibody [EPR8528]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8528

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not suitable for testing tissue samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab248361 is the carrier-free version of ab131159.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nicalin also known as NOMO1 is a protein involved in the Notch signaling pathway and has an approximate molecular mass of 70 kDa. This protein localizes predominantly in the endoplasmic reticulum. Nicalin expresses across multiple tissue types but shows higher levels in neural tissues. It interacts with Nomo1 and other proteins to form a multi-protein complex that modulates signaling functions.

Biological function summary

Nicalin acts as a regulator of Notch signaling an essential pathway for cell differentiation and development. It is a part of Nicalin-Nomo1 complex playing a significant role in modulating protein interactions. This complex contributes substantially to modifying cell fate decisions especially in the nervous system. Nicalin's presence in this complex highlights its importance in fine-tuning signaling mechanisms.

Pathways

Nicalin's function strongly influences Notch and Wnt signaling pathways. These pathways are important for processes such as cell proliferation and apoptosis. In the Notch pathway Nicalin forms interactions with the Presenilin protein influencing the maturation of Notch receptor proteins. This interaction affects cellular communication highlighting Nicalin's integrative role within signaling networks.

Nicalin shows a connection to neurodegenerative diseases like Alzheimer's Disease where its interaction with Presenilin is significant. The protein also relates to developmental disorders influencing nervous system development due to its role in the Notch signaling pathway. Nicalin's regulation within these pathways can impact disease progression and severity indicating its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed : 32820719, PubMed : 36261522). The MPT complex takes over after the SEC61 complex : following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed : 36261522). May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning, via its interaction with NOMO (By similarity).

See full target information NCLN

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicalin antibody [EPR8528] - BSA and Azide free

Western blot - Anti-Nicalin antibody [EPR8528] - BSA and Azide free (AB248361)

OI-RD Scanning - Anti-Nicalin antibody [EPR8528] - BSA and Azide free (AB248361)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise