AB3444

Anti-Nicastrin antibody

Name: Anti-Nicastrin antibody (ab3444) | Abcam
Brand: Abcam Limited
SKU: AB3444
Price: 510 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Nicastrin antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NCSTN aa 650 to C-terminus.

View Alternative Names

KIAA0253, UNQ1874/PRO4317, NCSTN, Nicastrin

3 Images

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Western blot - Anti-Nicastrin antibody (AB3444)

Western blot detection of Nicastrin on mouse primary cultured neuronal cells using ab3444.

All lanes:

Western blot - Anti-Nicastrin antibody (ab3444)

Predicted band size: 78 kDa

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Supplier Data

Western blot - Anti-Nicastrin antibody (AB3444)

All lanes:

Western blot - Anti-Nicastrin antibody (ab3444) at 1 µg/mL

Lane 1:

HeLa whole cell extract at 60 µg

Lane 2:

IMR-32 whole cell extract (human neuroblastoma cell line) at 60 µg

Lane 3:

NIH/3T3 whole cell extract (mouse embryo fibroblast cell line) at 60 µg

Lane 4:

L6 whole cell extract (Rat skeletal muscle cell line) at 60 µg

Lane 5:

mouse brain at 60 µg

Lane 6:

rat brain at 60 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 78 kDa

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Supplier Data

Western blot - Anti-Nicastrin antibody (AB3444)

All lanes:

Western blot - Anti-Nicastrin antibody (ab3444) at 2 µg/mL

Lane 1:

T98G membrane extract (Human glioblastoma cell line) at 30 µg

Lane 2:

RSC96 membrane extract (spontaneously immortalized rat Schwann cell line ) at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 78 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human NCSTN aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92542

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nicastrin also known as NAS is an integral component of the gamma-secretase complex. This protein interacts mechanically by assisting in substrate recognition and stabilization within the complex. Nicastrin has a molecular weight of approximately 75 kDa. It is predominantly expressed in various tissues including the brain where it plays significant role in neuronal functions.

Biological function summary

Nicastrin functions as a part of the gamma-secretase complex which also includes presenilin anterior pharynx defective 1 (APH-1) and presenilin enhancer 2 (PEN-2). This complex is essential for intramembrane proteolysis of type I membrane proteins such as amyloid precursor protein (APP) and Notch. The activity of nicastrin influences processes like synaptic organization and neuronal development.

Pathways

Gamma-secretase activity facilitated by nicastrin is central in the Notch signaling pathway and the amyloidogenic pathway. In the Notch pathway it helps release the Notch intracellular domain important for T-cell development. In the amyloidogenic pathway the complex processes APP contributing to amyloid-beta peptide formation. Beta-secretase (BACE1) also plays an important role in this pathway acting upstream of gamma-secretase.

The imbalance in nicastrin function connects it to Alzheimer's disease and certain cancers. Its role in amyloid-beta peptide production links nicastrin to Alzheimer's disease pathogenesis. In cancer the deregulation of Notch signaling in which nicastrin is involved influences tumor progression and survival. The interaction of nicastrin with presenilin is particularly notable in these conditions further emphasizing its significance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 10993067, PubMed : 12679784, PubMed : 25043039, PubMed : 26280335, PubMed : 30598546, PubMed : 30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.

See full target information NCSTN

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 43:102002 PubMed33984602

2021

Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Liu,Peixu Cong,Tao Zhang,Rui Wang,Xiaoxu Wang,Junyi Liu,Xinceng Wang,Jie Xu,Yuming Wang,Jingfeng Wang,Changhu Xue

Current Alzheimer research 10:4-10 PubMed22950863

2012

Evidence that enzyme processivity mediates differential Aβ production by PS1 and PS2.

Applications

Species

Unspecified reactive species

Sean A Pintchovski,Dale B Schenk,Guriqbal S Basi

Biology open 1:761-74 PubMed23213470

2012

Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Steuble,Tu-My Diep,Philipp Schätzle,Alexander Ludwig,Mitsuo Tagaya,Beat Kunz,Peter Sonderegger

The Biochemical journal 391:513-25 PubMed15975090

2005

Ubiquilin regulates presenilin endoproteolysis and modulates gamma-secretase components, Pen-2 and nicastrin.

Applications

Unspecified application

Species

Unspecified reactive species

Leann K Massey,Alex L Mah,Mervyn J Monteiro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicastrin antibody

Western blot - Anti-Nicastrin antibody (AB3444)

Western blot - Anti-Nicastrin antibody (AB3444)

Western blot - Anti-Nicastrin antibody (AB3444)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise