AB45425

Anti-Nicastrin antibody

Name: Anti-Nicastrin antibody (ab45425) | Abcam
Brand: Abcam Limited
SKU: AB45425
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal Nicastrin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NCSTN.

View Alternative Names

KIAA0253, UNQ1874/PRO4317, NCSTN, Nicastrin

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nicastrin antibody (AB45425)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Nicastrin antibody (AB45425)

Immunofluorescence of Nicastrin in Human Brain cells using ab45425 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (AB45425)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (AB45425)

ab45425 at 2.5 μg/ml staining Nicastrin in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

IHC-P

AbReview34394****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (AB45425)

ab45425 staining Nicastrin in Human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Carl Hobbs

Supplier Data

Western blot - Anti-Nicastrin antibody (AB45425)

Lane 1:

Western blot - Anti-Nicastrin antibody (ab45425) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Nicastrin antibody (ab45425) at 1 µg/mL

All lanes:

Human brain tissue lysate

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human NCSTN. The exact immunogen used to generate this antibody is proprietary information.

Q92542

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nicastrin also known as NAS is an integral component of the gamma-secretase complex. This protein interacts mechanically by assisting in substrate recognition and stabilization within the complex. Nicastrin has a molecular weight of approximately 75 kDa. It is predominantly expressed in various tissues including the brain where it plays significant role in neuronal functions.

Biological function summary

Nicastrin functions as a part of the gamma-secretase complex which also includes presenilin anterior pharynx defective 1 (APH-1) and presenilin enhancer 2 (PEN-2). This complex is essential for intramembrane proteolysis of type I membrane proteins such as amyloid precursor protein (APP) and Notch. The activity of nicastrin influences processes like synaptic organization and neuronal development.

Pathways

Gamma-secretase activity facilitated by nicastrin is central in the Notch signaling pathway and the amyloidogenic pathway. In the Notch pathway it helps release the Notch intracellular domain important for T-cell development. In the amyloidogenic pathway the complex processes APP contributing to amyloid-beta peptide formation. Beta-secretase (BACE1) also plays an important role in this pathway acting upstream of gamma-secretase.

The imbalance in nicastrin function connects it to Alzheimer's disease and certain cancers. Its role in amyloid-beta peptide production links nicastrin to Alzheimer's disease pathogenesis. In cancer the deregulation of Notch signaling in which nicastrin is involved influences tumor progression and survival. The interaction of nicastrin with presenilin is particularly notable in these conditions further emphasizing its significance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed : 10993067, PubMed : 12679784, PubMed : 25043039, PubMed : 26280335, PubMed : 30598546, PubMed : 30630874). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.

See full target information NCSTN

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Autonomic neuroscience : basic & clinical 247:103085 PubMed37031474

2023

Gamma secretase activity modulates BMP-7-induced dendritic growth in primary rat sympathetic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Krystal Karunungan,Rachel H Garza,Ana Cristina Grodzki,Megan Holt,Pamela J Lein,Vidya Chandrasekaran

Dermatology (Basel, Switzerland) 239:60-71 PubMed35843211

2022

Effects of NCSTN Mutation on Hair Follicle Components in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Wei Shi,Wei Cao,Qing-Zan Zhao,Hong-Xing Yu,Si-Sen Zhang,Yi-Bin Hao

The British journal of dermatology 182:491-493 PubMed31421058

2019

A novel NCSTN missense mutation in the signal peptide domain causes hidradenitis suppurativa, which has features characteristic of an autoinflammatory keratinization disease.

Applications

Unspecified application

Species

Unspecified reactive species

T Takeichi,T Matsumoto,T Nomura,M Takeda,H Niwa,M Kono,H Shimizu,T Ogi,M Akiyama

Scientific reports 9:2827 PubMed30808940

2019

Chronic cerebral hypoperfusion shifts the equilibrium of amyloid β oligomers to aggregation-prone species with higher molecular weight.

Applications

Unspecified application

Species

Unspecified reactive species

Taro Bannai,Tatsuo Mano,Xigui Chen,Gaku Ohtomo,Ryo Ohtomo,Takeyuki Tsuchida,Kagari Koshi-Mano,Tadafumi Hashimoto,Hitoshi Okazawa,Takeshi Iwatsubo,Shoji Tsuji,Tatsushi Toda,Atsushi Iwata

International journal of oncology 53:266-274 PubMed29749468

2018

Inhibition of CD9 expression reduces the metastatic capacity of human hepatocellular carcinoma cell line MHCC97-H.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Lin,Shifang Peng,Yixuan Yang

Molecular brain 8:27 PubMed25910195

2015

Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease.

Applications

Dot, WB

Species

Mouse, Mouse

Yohan D'Souza,Ahmed Elharram,Raquel Soon-Shiong,R David Andrew,Brian M Bennett

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicastrin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nicastrin antibody (AB45425)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (AB45425)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicastrin antibody (AB45425)

Western blot - Anti-Nicastrin antibody (AB45425)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise