Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on human testis. The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded cell pellets labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a CHRNA1 his tag construct, no staining on (B) HEK-293T transfected with empty plasmid. The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on human skeletal muscle (PMID : 34228850). The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on human kidney. The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on rat testis. The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Low expression : confocal image showing no staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Confocal image showing positive staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Confocal image showing positive staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat testis (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Low expression : confocal image showing no staining on rat testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Low expression : confocal image showing no staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/100 dilution (5.04 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green). Low expression : confocal image showing no staining on rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on mouse skeletal muscle (PMID : 30089464). The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on mouse testis. The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on mouse kidney. The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : testis (PMID : 34228850). This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID : 29874875; PMID : 36634413; PMID : 1694127; PMID : 11836518; PMID : 7695910). Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 70 seconds.

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

• WB

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (AB308306)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : testis and kidney (PMID : 34228850). This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID : 29874875; PMID : 36634413; PMID : 1694127; PMID : 11836518; PMID : 7695910). Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 37 seconds.

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (ab308306) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Lane 5:

Rat testis tissue lysate at 20 µg

Lane 6:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false