Rabbit Recombinant Monoclonal Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat, Transfected cell line samples.
Images
![Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--western-blot-img408804.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408817.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408818.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408819.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408815.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (AB308307)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Transfected cell line | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Transfected cell line | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Transfected cell line | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Alternative names
Acetylcholine receptor subunit alpha, CHRNA1, ACHRA, CHNRA
Recommended products
Rabbit Recombinant Monoclonal Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat, Transfected cell line samples.
Alternative names
Acetylcholine receptor subunit alpha, CHRNA1, ACHRA, CHNRA
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR27397-4
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
The Nicotinic Acetylcholine Receptor alpha 1 also known as CHRNA1 is an essential component of the nicotinic acetylcholine receptor complex. This receptor an ion channel found in the neuromuscular junction is involved in the mechanical process of signal transmission between nerves and muscles. The protein facilitates the binding of acetylcholine a neurotransmitter which triggers an ion flow causing muscle contraction. The CHRNA1 subunit generally displays a mass of approximately 58 kDa and is widely expressed in muscle tissues.
- Biological function summary
The Nicotinic Acetylcholine Receptor alpha 1 has a fundamental role in neuromuscular communication. It is part of a pentameric complex in combination with other acetylcholine receptor subunits. When acetylcholine binds to the receptor the channel opens permitting cations like sodium and calcium to enter the muscle cell leading to depolarization and subsequent muscle contraction. This biological process is essential for voluntary muscle movement.
- Pathways
The Nicotinic Acetylcholine Receptor alpha 1 is involved in the neuromuscular signaling pathway. This pathway regulates muscle contraction by converting neural chemical signals into mechanical responses. In this pathway CHRNA1 interacts closely with proteins such as the acetylcholine ligand and acetylcholine receptor. These interactions ensure effective synaptic transmission at the neuromuscular junction contributing to muscle activation and relaxation cycles.
- Associated diseases and disorders
Nicotinic Acetylcholine Receptor alpha 1 is linked to conditions such as myasthenia gravis and congenital myasthenic syndromes. In myasthenia gravis autoantibodies targeting CHRNA1 hinder normal receptor function on muscles leading to weakness and fatigue. Additionally the protein is associated with congenital myasthenic syndromes where mutations can alter the receptor's function resulting in impaired neuromuscular transmission. The receptor's relationship with other proteins like the acetylcholine receptor and AchR protein plays an important role in these conditions often defining the severity and specific characteristics of the disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
17 product images
![Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--western-blot-img408804.jpg "Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: testis and kidney (PMID: 34228850).
This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID:29874875; PMID:36634413; PMID:1694127; PMID:11836518; PMID:7695910).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 37 seconds.All lanes: Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
Lane 5: Rat testis tissue lysate at 20 µg
Lane 6: Rat kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: testis and kidney (PMID: 34228850).
This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID:29874875; PMID:36634413; PMID:1694127; PMID:11836518; PMID:7695910).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 37 seconds.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408817.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining on human kidney.
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408818.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining on human testis.
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408819.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining on mouse testis.
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408815.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining on rat testis.
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408814.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded cell pellets labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a CHRNA1 his tag construct, no staining on (B) HEK-293T transfected with empty plasmid.
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408813.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Cytoplasmic staining on rat skeletal muscle.
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408816.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining on mouse kidney.
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408810.jpg "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Low expression: confocal image showing no staining on mouse kidney.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408812.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/2000 dilution (0.252 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Cytoplasmic staining on mouse skeletal muscle (PMID: 30089464).
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408805.jpg "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Confocal image showing positive staining on mouse skeletal muscle.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408811.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/500 dilution (1.008 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Cytoplasmic staining on human skeletal muscle (PMID:34228850).
The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408808.jpg "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Low expression: confocal image showing no staining on rat kidney.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).![Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--western-blot-img408803.jpg "Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: testis (PMID: 34228850).
This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID:29874875; PMID:36634413; PMID:1694127; PMID:11836518; PMID:7695910).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 70 seconds.All lanes: Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] (Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 100 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: testis (PMID: 34228850).
This antibody can only recognize the functional skeletal muscle-specific isoform (isoform 1) dimers of CHRNA1. Functional CHRNA1 can form dimers with delta subunits (CHRND) or epsilon subunits (CHRNE) (PMID:29874875; PMID:36634413; PMID:1694127; PMID:11836518; PMID:7695910).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 70 seconds.![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408806.jpg "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Confocal image showing positive staining on rat skeletal muscle.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408809.jpg "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Low expression: confocal image showing no staining on mouse testis.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408807.jpg "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)
This data was produced using Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306, the same clone but in a different formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat testis (fresh) tissue labeling Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 at 1/100 dilution (5.04 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL) (Green).
Low expression: confocal image showing no staining on rat testis.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] ab308306 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408814.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408813.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408816.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408810.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408812.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408805.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408811.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408808.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--western-blot-img408803.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408806.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408809.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
![Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307), expandable thumbnail](https://content.abcam.com/products/images/nicotinic-acetylcholine-receptor-alpha-1-chrna1-antibody-epr27397-4-bsa-and-azide-free-ab308307--immunohistochemistry-frozen-sections-img408807.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [EPR27397-4] - BSA and Azide free (ab308307)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com