JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB288434

Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192]

Be the first to review this product! Submit a review

|

(1 Publication)

Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (ab288434) is a rat monoclonal antibody detecting Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Recombinant format for unrivalled batch-batch consistency

View Alternative Names

ACHRA, CHNRA, CHRNA1, Acetylcholine receptor subunit alpha

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (AB288434)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (AB288434)

The image data was generated using the chimeric mouse version of the same antibody clone

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, stained for muscle acetylcholine receptor using ab288434 at 10μg/ml for 1 hr. Followed by Alexa Fluor® 488 secondary antibody (2 μg/ml), showing positive cytoplasmic staining.

The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab288434, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (ab288434) followed by Alexa Fluor® 488 secondary antibody.

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (AB288434)
  • WB

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (AB288434)

The image data was generated using the chimeric mouse version of the same antibody clone

Run on a 10% SDS PAGE gel.

ab288434 successfully detected AChR in mouse skeletal muscle tissue lysate. Though this antibody is targetted to the human AChR it also reacts with mouse AChR.

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (ab288434) at 0.03 µg/mL

All lanes:

Mouse Skeletal Muscle lysate at 35 µg

Secondary

All lanes:

anti-mouse IgG1 secondary antibody

Predicted band size: 54 kDa

Observed band size: 58.9 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

mAb 192

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CHRNA1.

P02708

Specificity

The antibody binds to human muscle AChR with a Kd of 10 pM, to mouse muscle AChR with a Kd of 50 pM, and to rat muscle AChR with a Kd of 646 nM

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

What is this antibody validated in?
Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 antibody [mAb 192] (ab288434) is a rat recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of Nicotinic Acetylcholine Receptor alpha 1/CHRNA1?
Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 [mAb 192] (ab288434) specifically detects a band for Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 (UniProt: P02708) at a molecular weight of 54kDa.

Other related products
We have a range of other formats of antibody clone [mAb 192] also available for your convenience: ab288434, Carrier free - ab307999
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 1 also known as CHRNA1 is an essential component of the nicotinic acetylcholine receptor complex. This receptor an ion channel found in the neuromuscular junction is involved in the mechanical process of signal transmission between nerves and muscles. The protein facilitates the binding of acetylcholine a neurotransmitter which triggers an ion flow causing muscle contraction. The CHRNA1 subunit generally displays a mass of approximately 58 kDa and is widely expressed in muscle tissues.
Biological function summary

The Nicotinic Acetylcholine Receptor alpha 1 has a fundamental role in neuromuscular communication. It is part of a pentameric complex in combination with other acetylcholine receptor subunits. When acetylcholine binds to the receptor the channel opens permitting cations like sodium and calcium to enter the muscle cell leading to depolarization and subsequent muscle contraction. This biological process is essential for voluntary muscle movement.

Pathways

The Nicotinic Acetylcholine Receptor alpha 1 is involved in the neuromuscular signaling pathway. This pathway regulates muscle contraction by converting neural chemical signals into mechanical responses. In this pathway CHRNA1 interacts closely with proteins such as the acetylcholine ligand and acetylcholine receptor. These interactions ensure effective synaptic transmission at the neuromuscular junction contributing to muscle activation and relaxation cycles.

Nicotinic Acetylcholine Receptor alpha 1 is linked to conditions such as myasthenia gravis and congenital myasthenic syndromes. In myasthenia gravis autoantibodies targeting CHRNA1 hinder normal receptor function on muscles leading to weakness and fatigue. Additionally the protein is associated with congenital myasthenic syndromes where mutations can alter the receptor's function resulting in impaired neuromuscular transmission. The receptor's relationship with other proteins like the acetylcholine receptor and AchR protein plays an important role in these conditions often defining the severity and specific characteristics of the disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Isoform 1. Upon acetylcholine binding, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.. Isoform 2. Non functional acetylcholine receptor alpha subunit which is not integrated into functional acetylcholine-gated cation-selective channels.
See full target information CHRNA1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FEBS letters 389:195-8 PubMed8766828

1996

Characterisation, crystallisation and preliminary X-ray diffraction analysis of a Fab fragment of a rat monoclonal antibody with very high affinity for the human muscle acetylcholine receptor.

Applications

Unspecified application

Species

Unspecified reactive species

M Kontou,E H Vatzaki,A Kokla,K R Acharya,N G Oikonomakos,S J Tzartos
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB76159

Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y]

RabMAb|Recombinant

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (AB76159)

  • 0
  • 0
Primary Antibodies

AB124832

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (AB124832)

  • 4
  • 2
Primary Antibodies

AB37484

Anti-Myosin heavy chain antibody [A4.1025]

BOND RX™ Validated|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] (AB37484)

  • 0
  • 0
Primary Antibodies

AB216485

Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (AB216485)

  • 4
  • 5

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com