AB151580

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody

Name: Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab151580) | Abcam
Brand: Abcam Limited
SKU: AB151580
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NACHRA3, CHRNA3, Neuronal acetylcholine receptor subunit alpha-3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (AB151580)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (AB151580)

Immunofluorescence analysis of methanol-fixed A431 cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 with ab151580 1/500 dilution.

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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (AB151580)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab151580) at 1/1000 dilution

All lanes:

Molt-4 cell lysate at 30 µg

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 3 also known as CHRNA3 is a subunit of the nicotinic acetylcholine receptors important for mediating synaptic transmission. This protein with a mass of approximately 57 kDa forms one part of a pentameric complex which functions as an ion channel. It is widely expressed in the central and peripheral nervous systems contributing to the signaling mechanisms in neurons. CHRNA3 in conjunction with other nicotinic receptor subunits plays a role in neurotransmitter release exerting control over calcium and sodium ion influx that affects neuron excitability and response.

Biological function summary

CHRNA3 serves as a critical element of neurotransmission playing a part in the nicotine-induced dopaminergic signaling pathways. Often forming a complex with other subunits like beta-2 and beta-4 it modulates synaptic plasticity and influences behavioral processes such as addiction and attention. This subunit composition affects receptor specificity and sensitivity determining how neurons respond to acetylcholine and nicotine.

Pathways

The involvement of CHRNA3 in cholinergic signaling pathways is significant. It contributes to the role of nicotinic acetylcholine receptors in the addictive properties of substances like nicotine. Interaction with proteins such as CHRNB2 (nicotinic acetylcholine receptor beta-2 subunit) exemplifies how CHRNA3 participates in modulating synaptic strength and integration in the central nervous system. Its regulatory activity in these pathways demonstrates its importance in maintaining the balance of neurotransmitter systems.

Aberrations in CHRNA3 expression or function link to several neuropsychiatric and neurodegenerative conditions. It associates with nicotine dependence and lung cancer where alterations in receptor activity influence disease progression. In these contexts CHRNA3 interacts with proteins like CHRNB4 further implicating its role in pathological nicotine dependence and suggesting potential for targeted intervention strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

See full target information CHRNA3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 14:485-501 PubMed37204253

2023

Role of creatine shuttle in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mayu Kita,Rina Fujiwara-Tani,Shingo Kishi,Shiori Mori,Hitoshi Ohmori,Chie Nakashima,Kei Goto,Takamitsu Sasaki,Kiyomu Fujii,Isao Kawahara,Ujjal Kumar Bhawal,Yi Luo,Hiroki Kuniyasu

International journal of molecular sciences 23: PubMed36012367

2022

α3β4 Acetylcholine Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells.

Applications

Unspecified application

Species

Unspecified reactive species

José Villanueva,Manuel Criado,Yolanda Giménez-Molina,Virginia González-Vélez,Amparo Gil,Luis Miguel Gutiérrez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (AB151580)

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (AB151580)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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