AB41172

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody

Name: Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172) | Abcam
Brand: Abcam Limited
SKU: AB41172
Price: 430 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Pufferfish, Snail, Zebrafish, Chicken, Chimpanzee, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CHRNA4 aa 600 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NACRA4, CHRNA4, Neuronal acetylcholine receptor subunit alpha-4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (AB41172)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (AB41172)

ab41172 (1/250 dilution) staining Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and membrane compartment within the glia region of cells
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (AB41172)

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (ab41172) at 1/800 dilution

Lane 1:

SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pufferfish, Chicken, Snail, Chimpanzee, Zebrafish, Rat, Mouse

Applications

ELISA, ICC/IF, IHC-Fr, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CHRNA4 aa 600 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P43681

Specificity

Antiserum Specificity Polypeptide % Cross Reactivity Nicotinic a4 receptor (620-627) 100 Nicotinic a4 receptor ~80 Nicotinic a3 receptor 0 Nicotinic a5 receptor 0 Nicotinic a7 receptor 0 Nicotinic ß2 receptor 0 Nicotinic ß3 receptor 0 Nicotinic ß4 receptor 0

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 4 subunit also known as CHRNA4 forms a part of the nicotinic cholinergic receptor family. It serves as a ligand-gated ion channel responsive to the neurotransmitter acetylcholine. This receptor's proper function involves the binding of acetylcholine which results in the opening of an ion channel leading to ion flow across the membrane. CHRNA4 has a molecular mass of about 67 kDa and typically pairs with other subunits like beta 2 (β2) to form a functional receptor. It is primarily expressed in the central nervous system particularly within the cerebral cortex hippocampus and basal forebrain regions.

Biological function summary

CHRNA4's role extends beyond the basic mechanism of ion channel opening. It engages in the modulation of synaptic activity and participates in the cognitive processes due to its localization in the brain. CHRNA4 usually forms a receptor complex with other types of alpha and beta subunits such as the alpha 3 and beta 2 subunits contributing to the diversity due to its different subunit composition. This variety of subunit combinations determines distinct pharmacological and kinetic properties of the resulting ion channels.

Pathways

The CHRNA4 subunit involved in the neurotransmission pathways integrates into the cholinergic signaling pathway. This pathway is essential for modulating various cognitive functions including learning and memory. The CHRNA4 subunit closely interacts with other proteins such as CHRNB2 as part of the receptor complex functioning in the cholinergic synaptic transmission. This interaction supports the involvement in key neurotransmitter systems that regulate mood arousal and cognitive function.

Aberrations in CHRNA4 function link to several neurological conditions. It associates strongly with autosomal dominant nocturnal frontal lobe epilepsy where mutations in CHRNA4 can lead to hypersynchronization of neuronal activity. Additionally alterations in this receptor are implicated in nicotine addiction where genetic variations in CHRNA4 influence vulnerability. These disorders illustrate the broader role of CHRNA4 within the brain's network emphasizing its connection to other cholinergic receptors and proteins such as CHRNB2 which also share involvement in these disease pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.

See full target information CHRNA4

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biology of reproduction 111:472-482 PubMed38713677

2024

Neonicotinoids differentially modulate nicotinic acetylcholine receptors in immature and antral follicles in the mouse ovary†.

Applications

Unspecified application

Species

Unspecified reactive species

Vasiliki E Mourikes,Ramsés Santacruz-Márquez,Ashley Deviney,Alison Neff,Mary J Laws,Jodi A Flaws

Molecular neurobiology 59:3280-3293 PubMed35297011

2022

Nicotine Activating αβ Nicotinic Acetylcholine Receptors to Suppress Neuroinflammation via JAK2-STAT3 Signaling Pathway in Ischemic Rats and Inflammatory Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Jinyu Gou,Shenrui Guo,Feng Wei,Tingting Han,Ruihe Lai,Dalong Zhang,Yao Diao,Yafu Yin

Nature communications 10:4037 PubMed31492869

2019

Central nicotine induces browning through hypothalamic κ opioid receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Seoane-Collazo,Laura Liñares-Pose,Eva Rial-Pensado,Amparo Romero-Picó,José María Moreno-Navarrete,Noelia Martínez-Sánchez,Pablo Garrido-Gil,Ramón Iglesias-Rey,Donald A Morgan,Naoki Tomasini,Samuel Andrew Malone,Ana Senra,Cintia Folgueira,Gema Medina-Gomez,Tomás Sobrino,José L Labandeira-García,Rubén Nogueiras,Ana I Domingos,José-Manuel Fernández-Real,Kamal Rahmouni,Carlos Diéguez,Miguel López

Substance abuse : research and treatment 12:1178221818801316 PubMed31068752

2018

Influence of Tobacco Smoke Exposure on the Protein Expression of α7 and α4 Nicotinic Acetylcholine Receptors in Squamous Cell Carcinoma Tumors of the Upper Aerodigestive Tract (Out of the Larynx).

Applications

Unspecified application

Species

Unspecified reactive species

Bertha B Montaño-Velázquez,Juan C Benavides Méndez,Francisco J García-Vázquez,Ernesto Conde-Vázquez,Magdalena Sánchez-Uribe,Cecilia R Taboada-Murrieta,Kathrine Jáuregui-Renaud

Nature neuroscience 18:892-902 PubMed25915477

2015

An acetylcholine-activated microcircuit drives temporal dynamics of cortical activity.

Applications

IHC

Species

Unspecified reactive species

Naiyan Chen,Hiroki Sugihara,Mriganka Sur

Neurotoxicology 35:41-9 PubMed23261423

2012

3,4-Methylenedioxy-methamphetamine induces in vivo regional up-regulation of central nicotinic receptors in rats and potentiates the regulatory effects of nicotine on these receptors.

Applications

Unspecified application

Species

Unspecified reactive species

David Pubill,Sara Garcia-Ratés,Jordi Camarasa,Elena Escubedo

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (AB41172)

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody (AB41172)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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