AB124832

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)]

Name: Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (ab124832) | Abcam
Brand: Abcam Limited
SKU: AB124832
Price: 180 USD
Availability: InStock
Rating: 4 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

NACRA4, CHRNA4, Neuronal acetylcholine receptor subunit alpha-4

1 Images

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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (AB124832)

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (ab124832) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

A673 cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

Neuro-2a cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

Carrier free

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4563(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 4 subunit also known as CHRNA4 forms a part of the nicotinic cholinergic receptor family. It serves as a ligand-gated ion channel responsive to the neurotransmitter acetylcholine. This receptor's proper function involves the binding of acetylcholine which results in the opening of an ion channel leading to ion flow across the membrane. CHRNA4 has a molecular mass of about 67 kDa and typically pairs with other subunits like beta 2 (β2) to form a functional receptor. It is primarily expressed in the central nervous system particularly within the cerebral cortex hippocampus and basal forebrain regions.

Biological function summary

CHRNA4's role extends beyond the basic mechanism of ion channel opening. It engages in the modulation of synaptic activity and participates in the cognitive processes due to its localization in the brain. CHRNA4 usually forms a receptor complex with other types of alpha and beta subunits such as the alpha 3 and beta 2 subunits contributing to the diversity due to its different subunit composition. This variety of subunit combinations determines distinct pharmacological and kinetic properties of the resulting ion channels.

Pathways

The CHRNA4 subunit involved in the neurotransmission pathways integrates into the cholinergic signaling pathway. This pathway is essential for modulating various cognitive functions including learning and memory. The CHRNA4 subunit closely interacts with other proteins such as CHRNB2 as part of the receptor complex functioning in the cholinergic synaptic transmission. This interaction supports the involvement in key neurotransmitter systems that regulate mood arousal and cognitive function.

Aberrations in CHRNA4 function link to several neurological conditions. It associates strongly with autosomal dominant nocturnal frontal lobe epilepsy where mutations in CHRNA4 can lead to hypersynchronization of neuronal activity. Additionally alterations in this receptor are implicated in nicotine addiction where genetic variations in CHRNA4 influence vulnerability. These disorders illustrate the broader role of CHRNA4 within the brain's network emphasizing its connection to other cholinergic receptors and proteins such as CHRNB2 which also share involvement in these disease pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed : 22361591, PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits. Is the most abundant nAChR subtype expressed in the central nervous system (PubMed : 16835356, PubMed : 22361591, PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). Found in two major stoichiometric forms,(CHRNA4)3 : (CHRNB2)2 and (CHRNA4)2 : (CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). Involved in the modulation of calcium-dependent signaling pathways, influences the release of neurotransmitters, including dopamine, glutamate and GABA (By similarity).

See full target information CHRNA4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 18: PubMed31963558

2020

Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Sun,Manqi Zhangsun,Shuai Dong,Yiqiao Liu,Jiang Qian,Dongting Zhangsun,Sulan Luo

Marine drugs 17: PubMed31035425

2019

Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiao Liu,Jiang Qian,Zhihua Sun,Dongting Zhangsun,Sulan Luo

Scientific reports 6:27283 PubMed27277673

2016

Accumulation of human full-length tau induces degradation of nicotinic acetylcholine receptor α4 via activating calpain-2.

Applications

Species

Unspecified reactive species

Yaling Yin,Yali Wang,Di Gao,Jinwang Ye,Xin Wang,Lin Fang,Dongqin Wu,Guilin Pi,Chengbiao Lu,Xin-Wen Zhou,Ying Yang,Jian-Zhi Wang

Behavioral and brain functions : BBF 12:13 PubMed27068709

2016

Involvement of hippocampal acetylcholinergic receptors in electroacupuncture analgesia in neuropathic pain rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Ping Chen,Yu Kan,Jian Liang Zhang,Jun Ying Wang,Yong Hui Gao,Li Na Qiao,Xiu Mei Feng,Ya Xia Yan,Jun Ling Liu

Progress in neuro-psychopharmacology & biological psychiatry 53:1-8 PubMed24614096

2014

Protracted treatment with MDMA induces heteromeric nicotinic receptor up-regulation in the rat brain: an autoradiography study.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Ciudad-Roberts,Jorge Camarasa,David Pubill,Elena Escubedo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)]

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (AB124832)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise