AB240004

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

NACRA4, CHRNA4, Neuronal acetylcholine receptor subunit alpha-4

1 Images

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Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free (AB240004)

This data was developed using ab124832, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] (<a href='/en-us/products/primary-antibodies/nicotinic-acetylcholine-receptor-alpha-4-chrna4-antibody-epr45632-ab124832'>ab124832</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

A673 cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

Neuro-2a cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

Unconjugated

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4563(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240004 is the carrier-free version of ab124832.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 4 subunit also known as CHRNA4 forms a part of the nicotinic cholinergic receptor family. It serves as a ligand-gated ion channel responsive to the neurotransmitter acetylcholine. This receptor's proper function involves the binding of acetylcholine which results in the opening of an ion channel leading to ion flow across the membrane. CHRNA4 has a molecular mass of about 67 kDa and typically pairs with other subunits like beta 2 (β2) to form a functional receptor. It is primarily expressed in the central nervous system particularly within the cerebral cortex hippocampus and basal forebrain regions.

Biological function summary

CHRNA4's role extends beyond the basic mechanism of ion channel opening. It engages in the modulation of synaptic activity and participates in the cognitive processes due to its localization in the brain. CHRNA4 usually forms a receptor complex with other types of alpha and beta subunits such as the alpha 3 and beta 2 subunits contributing to the diversity due to its different subunit composition. This variety of subunit combinations determines distinct pharmacological and kinetic properties of the resulting ion channels.

Pathways

The CHRNA4 subunit involved in the neurotransmission pathways integrates into the cholinergic signaling pathway. This pathway is essential for modulating various cognitive functions including learning and memory. The CHRNA4 subunit closely interacts with other proteins such as CHRNB2 as part of the receptor complex functioning in the cholinergic synaptic transmission. This interaction supports the involvement in key neurotransmitter systems that regulate mood arousal and cognitive function.

Aberrations in CHRNA4 function link to several neurological conditions. It associates strongly with autosomal dominant nocturnal frontal lobe epilepsy where mutations in CHRNA4 can lead to hypersynchronization of neuronal activity. Additionally alterations in this receptor are implicated in nicotine addiction where genetic variations in CHRNA4 influence vulnerability. These disorders illustrate the broader role of CHRNA4 within the brain's network emphasizing its connection to other cholinergic receptors and proteins such as CHRNB2 which also share involvement in these disease pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.

See full target information CHRNA4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free

Western blot - Anti-Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 antibody [EPR4563(2)] - BSA and Azide free (AB240004)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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