AB110851

Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

Name: Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (ab110851) | Abcam
Brand: Abcam Limited
SKU: AB110851
Rating: 1 (1 reviews)

(1 Review)

(3 Publications)

Goat Polyclonal Nicotinic Acetylcholine Receptor alpha 7 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CHRNA7 aa 300-400.

View Alternative Names

NACHRA7, CHRNA7, Neuronal acetylcholine receptor subunit alpha-7

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (AB110851)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (AB110851)

ab110851 at 5μg/ml staining Nicotinic Acetylcholine Receptor alpha 7 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CHRNA7 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P36544

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99.48% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor alpha 7 (nAChR α7) is an important ligand-gated ion channel involved in neurotransmission. It is commonly known by its alternate name α7-nAChR. This receptor plays a critical role in fast synaptic transmission and is activated by the neurotransmitter acetylcholine. The nAChR α7 is a homomeric receptor meaning it is composed entirely of alpha 7 subunits each with a molecular mass of approximately 57 kDa. It is expressed predominantly in the central nervous system especially in the brain but also found in other tissues like the immune system.

Biological function summary

The nAChR α7 facilitates the influx of cations like calcium and sodium upon activation influencing cellular signaling events. As part of the larger family of nicotinic acetylcholine receptors it functions within a pentameric complex structure. The receptor is essential for cognitive processes including attention and memory by modulating neurotransmitter release and plasticity. It also interacts with other neurotransmitter pathways and may play a role in anti-inflammatory processes due to its expression in non-neuronal tissues.

Pathways

The nAChR α7 is involved in the cholinergic signaling pathway and the broader calcium signaling pathway. It contributes to the regulation of intracellular calcium levels which are important for various cellular functions. The receptor works alongside other proteins such as bungarotoxin-sensitive nAChRs and muscarinic acetylcholine receptors (mAChRs) to mediate complex neurotransmitter systems. The interplay between these receptors highlights the receptor's importance in maintaining synaptic function and plasticity.

Research indicates a connection between nAChR α7 and neurodegenerative diseases like Alzheimer’s disease and schizophrenia. The receptor's dysfunction or altered expression has been linked to cognitive deficits associated with these conditions. In Alzheimer's disease the interaction of nAChR α7 with amyloid-beta peptides may contribute to neurotoxicity. Additionally the receptor's association with cytokine release in immune cells suggests a potential role in inflammatory disorders. Its linkage with other neurotransmitter systems such as the involvement of nAChR agonists further demonstrates the receptor's relevance to these neurological and immune-associated diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

See full target information CHRNA7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 12:706225 PubMed34248648

2021

Cytisine Exerts an Anti-Epileptic Effect via α7nAChRs in a Rat Model of Temporal Lobe Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Jun Zheng,Teng-Yue Zhang,Hong-Tao Liu,Ze-Xin Huang,Jing-Mei Teng,Jing-Xian Deng,Jia-Gui Zhong,Xu Qian,Xin-Wen Sheng,Ji-Qiang Ding,Shu-Qiao He,Xin Zhao,Wei-Dong Ji,De-Feng Qi,Wei Li,Mei Zhang

Frontiers in immunology 12:631881 PubMed33815383

2021

New Pathways for the Skin's Stress Response: The Cholinergic Neuropeptide SLURP-1 Can Activate Mast Cells and Alter Cytokine Production in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph M Ertle,Frank R Rommel,Susanne Tumala,Yasuhiro Moriwaki,Jochen Klein,Johannes Kruse,Uwe Gieler,Eva M J Peters

EBioMedicine 45:495-510 PubMed31253515

2019

Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Schneider Nunes,Parwathy Chandran,Maggie Sundby,Fernanda Visioli,Fabiany da Costa Gonçalves,Scott Robert Burks,Ana Helena Paz,Joseph Alan Frank

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (AB110851)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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