AB189174

Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody

Name: Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab189174) | Abcam
Brand: Abcam Limited
SKU: AB189174
Price: 630 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHRNB2 aa 350-400.

View Alternative Names

Neuronal acetylcholine receptor subunit beta-2, CHRNB2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (AB189174)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (AB189174)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling Nicotinic Acetylcholine Receptor beta 2/CHRNB2 with ab189174 at 3.75 μg/ml.

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (AB189174)

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab189174) at 1 µg/mL

All lanes:

Human cerebellum lysate (in RIPA buffer) at 35 µg

Predicted band size: 57 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CHRNB2 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P17787

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor beta 2 (CHRNB2) also known as nAChR beta-2 subunit is an integral membrane protein involved in forming ligand-gated ion channels. It has a pivotal role in neurotransmission by responding to the neurotransmitter acetylcholine. CHRNB2 typically forms heteromeric complexes with alpha subunits to yield functional receptors. It is highly expressed in the central nervous system including regions such as the cerebral cortex and hippocampus. Each beta-2 subunit has a molecular mass of approximately 50 kDa.

Biological function summary

CHRNB2 influences synaptic plasticity and cognitive processes. It functions as part of a pentameric receptor complex usually composed of various subunits that determine the receptor's physiological properties. The receptor modulates neurotransmitter release and impacts neuronal excitability. Its activity contributes to cognitive functions such as attention learning and memory mediated through ion flow across cell membranes that affect neuron firing rates.

Pathways

The beta-2 subunit of the nicotinic acetylcholine receptor is integral to the cholinergic pathway. It interacts with the dopaminergic system by modulating dopaminergic neuron activity and dopamine release. This interaction plays a significant role in pathways related to reward pleasure and addiction. Proteins such as the dopamine transporter (DAT) are closely linked within these pathways affecting the dynamics of neurotransmitter availability and receptor activation.

Alterations in CHRNB2 function associate with neurological disorders like nicotine addiction and Alzheimer's disease. Dysfunctional receptors due to mutations can disrupt cognitive function and behavior highlighting their role in Alzheimer's pathology where neuronal communication is impaired. Other proteins such as amyloid precursor protein (APP) link CHRNB2 to the disease process by influencing neurodegenerative outcomes and synaptic maintenance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed : 22361591, PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). CHRNB2 forms heteropentameric neuronal acetylcholine receptors with CHRNA2, CHRNA3, CHRNA4 and CHRNA6, as well as CHRNA5 and CHRNB3 as accesory subunits (PubMed : 16835356, PubMed : 20881005, PubMed : 22361591, PubMed : 27698419, PubMed : 29720657, PubMed : 38454578, PubMed : 8663494). Found in two major stoichiometric forms,(CHRNA4)3 : (CHRNB2)2 and (CHRNA4)2 : (CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed : 27698419, PubMed : 29720657, PubMed : 38454578). Heteropentameric channels with CHRNA6 and CHRNA4 exhibit high sensitivity to ACh and nicotine and are predominantly expressed in only a few brain areas, including dopaminergic neurons, norepirephrine neurons and cells of the visual system. nAChrs containing CHRNA6 subunits mediate endogenous cholinergic modulation of dopamine and gamma-aminobutyric acid (GABA) release in response to nicotine at nerve terminals (By similarity). Also forms functional nAChRs with other subunits such as CHRNA7 : CHRNB2, mainly expressed in basal forebrain cholinergic neurons (PubMed : 33239400, PubMed : 38161283).

See full target information CHRNB2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 59:3280-3293 PubMed35297011

2022

Nicotine Activating αβ Nicotinic Acetylcholine Receptors to Suppress Neuroinflammation via JAK2-STAT3 Signaling Pathway in Ischemic Rats and Inflammatory Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Jinyu Gou,Shenrui Guo,Feng Wei,Tingting Han,Ruihe Lai,Dalong Zhang,Yao Diao,Yafu Yin

Scientific reports 9:3805 PubMed30846819

2019

Direct Effects of Nicotine Exposure on Murine Calvaria and Calvarial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Durham,R Nicole Howie,Graham Warren,Amanda LaRue,James Cray

View all publications

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Product promise

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Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (AB189174)

Western blot - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (AB189174)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise