Rabbit Polyclonal Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
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- Cancer cell international 22:3402022CHRNB2 represses pancreatic cancer migration and invasion via inhibiting β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Qin,Tianhao Li,Yuanyang Wang,Bangbo Zhao,Zeru Li,Tianyu Li,Xiaoying Yang,Yutong Zhao,Weibin WangPubMed 36344976
- Nature communications 10:40372019Central nicotine induces browning through hypothalamic κ opioid receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia Seoane-Collazo et. al.PubMed 31492869
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS, Whole serum, 1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions.
Alternative names
Neuronal acetylcholine receptor subunit beta-2, CHRNB2
Recommended products
Rabbit Polyclonal Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS, Whole serum, 1% BSA
- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purity
Whole antiserum
- Reconstitution
Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent of whole antiserum
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)
ICC/IF image of ab41174 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41174, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174)
The band observed at 52 kDa could potentially be a cleaved form of Nicotinic Acetylcholine Receptor beta 2/CHRNB2 due to the presence of a 25 amino acid signal peptide.
All lanes: Western blot - Anti-Nicotinic Acetylcholine Receptor beta 2/CHRNB2 antibody (ab41174) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 46 kDa, 52 kDa
Exposure time: 3min
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